蓝藻生物钟蛋白KaiC的表达、纯化及其自磷酸酶活性的测定
Expression and Purification of Cyanobacterial Circadian Clock Protein KaiC and Determination of Its Auto-phosphatase Activity.
作者信息
Chen Qiang, Yu Lingling, Tan Xiao, Liu Sen
机构信息
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China.
College of Medical Science, China Three Gorges University, Yichang, China.
出版信息
Bio Protoc. 2017 Feb 20;7(4):e2140. doi: 10.21769/BioProtoc.2140.
Abstract
Circadian rhythms are biological processes displaying an endogenous oscillation with a period of ~24 h. They allow organisms to anticipate and get prepared for the environmental changes caused mainly by the rotation of Earth. Circadian rhythms are driven by circadian clocks that consist of proteins, DNA, and/or RNA. Circadian clocks of cyanobacteria are the simplest and one of the best studied models. They contain the three clock proteins KaiA, KaiB, and KaiC which can be used for reconstitution experiments and determination of the auto-phosphatase activity of KaiC as described in this protocol.
摘要
昼夜节律是一种生物过程，呈现出约24小时周期的内源性振荡。它们使生物体能够预测并为主要由地球自转引起的环境变化做好准备。昼夜节律由生物钟驱动，生物钟由蛋白质、DNA和/或RNA组成。蓝细菌的生物钟是最简单且研究得最透彻的模型之一。它们包含三种生物钟蛋白KaiA、KaiB和KaiC，如本方案所述，这些蛋白可用于重组实验和测定KaiC的自磷酸酶活性。
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