Zhu Chun-Quan, Zhu Xiao-Fang, Shen Ren-Fang
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.
Bio Protoc. 2017 Feb 20;7(4):e2141. doi: 10.21769/BioProtoc.2141.
Organic acids secreted from plant roots play important roles in various biological processes including nutrient acquisition, metal detoxification, and pathogen attraction. The secretion of organic acids may be affected by various conditions such as plant growth stage, nutrient deficiency, and abiotic stress. For example, when white lupin ( L.) is exposed to phosphorus (P)-deficient conditions, the secretion of citrate acid from its proteoid roots significantly increases ( Neumann , 1999 ). This protocol describes a method for the collection and measurement of the efflux of organic acids (oxalate, malate, and citrate) from the roots of rice cultivar Nipponbare ('Nip') under different nitrogen forms (NH and NO-), together with different P supply (+P and -P) conditions.
植物根系分泌的有机酸在多种生物学过程中发挥着重要作用，包括养分获取、金属解毒和病原体吸引。有机酸的分泌可能受到多种条件的影响，如植物生长阶段、养分缺乏和非生物胁迫。例如，当白羽扇豆暴露于缺磷条件下时，其排根中柠檬酸的分泌显著增加（诺伊曼，1999年）。本方案描述了一种在不同氮形态（NH 和NO-）以及不同磷供应（+P和-P）条件下，收集和测量水稻品种日本晴（‘Nip’）根系有机酸（草酸盐、苹果酸盐和柠檬酸盐）外排的方法。
