RNA诱导的PKR激活的测量

Measurement of RNA-induced PKR Activation .

作者信息

Yuen Kobe C

机构信息

Stowers Institute for Medical Research, Kansas City, MO, USA.

Present address: 1 DNA Way, South San Francisco, USA.

出版信息

Bio Protoc. 2017 Mar 20;7(6):e2178. doi: 10.21769/BioProtoc.2178.

DOI:10.21769/BioProtoc.2178

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8376569/

Abstract

Protein kinase R (PKR) is one of the key RNA-activated sensors for innate immunity. PKR is activated by pathogenic or aberrant RNAs such as short double-stranded RNAs or those with imperfect secondary structures, as well as a reduction in the amount and number of RNA modifications. Activation of PKR may be an underlying mechanism for the pathogenesis of human diseases. In this protocol, I describe a method for studying levels of RNA-induced PKR activation .

摘要

蛋白激酶R（PKR）是先天性免疫的关键RNA激活传感器之一。PKR可被致病性或异常RNA激活，如短双链RNA或二级结构不完善的RNA，以及RNA修饰的数量和含量减少。PKR的激活可能是人类疾病发病机制的潜在机制。在本方案中，我描述了一种研究RNA诱导的PKR激活水平的方法。

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本文引用的文献

1

NIPBL Controls RNA Biogenesis to Prevent Activation of the Stress Kinase PKR.

Cell Rep. 2016 Jan 5;14(1):93-102. doi: 10.1016/j.celrep.2015.12.012. Epub 2015 Dec 24.

2

Nucleoside modifications modulate activation of the protein kinase PKR in an RNA structure-specific manner.

RNA. 2008 Jun;14(6):1201-13. doi: 10.1261/rna.1007408. Epub 2008 Apr 21.

3

eIF2alpha kinases provide a new solution to the puzzle of substrate specificity.

Nat Struct Mol Biol. 2005 Oct;12(10):835-8. doi: 10.1038/nsmb1005-835.

4

Translational control in stress and apoptosis.

Nat Rev Mol Cell Biol. 2005 Apr;6(4):318-27. doi: 10.1038/nrm1618.

5

Activation of the protein kinase PKR by short double-stranded RNAs with single-stranded tails.

RNA. 2004 Dec;10(12):1934-45. doi: 10.1261/rna.7150804.

6

Binding of the protein kinase PKR to RNAs with secondary structure defects: role of the tandem A-G mismatch and noncontiguous helixes.

Biochemistry. 1998 May 5;37(18):6303-16. doi: 10.1021/bi980113j.

7

Interactions between double-stranded RNA regulators and the protein kinase DAI.

Mol Cell Biol. 1992 Nov;12(11):5238-48. doi: 10.1128/mcb.12.11.5238-5248.1992.

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