RNA诱导的PKR激活的测量
Measurement of RNA-induced PKR Activation .
作者信息
Yuen Kobe C
机构信息
Stowers Institute for Medical Research, Kansas City, MO, USA.
Present address: 1 DNA Way, South San Francisco, USA.
出版信息
Bio Protoc. 2017 Mar 20;7(6):e2178. doi: 10.21769/BioProtoc.2178.
Abstract
Protein kinase R (PKR) is one of the key RNA-activated sensors for innate immunity. PKR is activated by pathogenic or aberrant RNAs such as short double-stranded RNAs or those with imperfect secondary structures, as well as a reduction in the amount and number of RNA modifications. Activation of PKR may be an underlying mechanism for the pathogenesis of human diseases. In this protocol, I describe a method for studying levels of RNA-induced PKR activation .
摘要
蛋白激酶R（PKR）是先天性免疫的关键RNA激活传感器之一。PKR可被致病性或异常RNA激活，如短双链RNA或二级结构不完善的RNA，以及RNA修饰的数量和含量减少。PKR的激活可能是人类疾病发病机制的潜在机制。在本方案中，我描述了一种研究RNA诱导的PKR激活水平的方法。
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