Department of Genetics and Breeding Biology, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976, Nitra, Slovakia.
Department of Plant Physiology, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976, Nitra, Slovakia.
World J Microbiol Biotechnol. 2021 Aug 30;37(10):165. doi: 10.1007/s11274-021-03130-2.
In our study we investigated the effect of different nickel (NiSO·6HO) (Ni) concentrations on cell division, cellular morphology and ionome homeostasis of the eukaryotic model organism Schizosaccharomyces pombe. Target of rapamycin (TOR) protein kinase is one of the key regulators of cell growth under different environmental stresses. We analyzed the effect of Ni on cell strains lacking the Tor1 signaling pathway utilizing light-absorbance spectroscopy, visualization, microscopy and inductively coupled plasma optical emission spectroscopy. Interestingly, our findings revealed that Ni mediated cell growth alterations are noticeably lower in Tor1 deficient cells. Greater size of Tor1 depleted cells reached similar quantitative parameters to wild type cells upon incubation with 400 μM Ni. Differences of ion levels among the two tested yeast strains were detected even before Ni addition. Addition of high concentration (1 mM) of the heavy metal, representing acute contamination, caused considerable changes in the ionome of both strains. Strikingly, Tor1 deficient cells displayed largely reduced Ni content after treatment compared to wild type controls (644.1 ± 49 vs. 2096.8 ± 75 μg/g), suggesting its significant role in Ni trafficking. Together our results predict yet undefined role for the Tor1 signaling in metal uptake and/or metabolism.
在我们的研究中，我们调查了不同浓度的镍（NiSO·6HO）（Ni）对真核模式生物裂殖酵母细胞分裂、细胞形态和离子组稳态的影响。雷帕霉素（TOR）蛋白激酶是不同环境应激下细胞生长的关键调节剂之一。我们利用光吸收光谱、可视化、显微镜和电感耦合等离子体发射光谱分析了 Ni 对缺乏 Tor1 信号通路的细胞株的影响。有趣的是，我们的发现表明，在 Tor1 缺失的细胞中，Ni 介导的细胞生长变化明显较低。在 400 μM Ni 孵育下，Tor1 耗尽细胞的体积增大，达到与野生型细胞相似的定量参数。在添加 Ni 之前，就已经检测到两种测试酵母菌株之间的离子水平存在差异。添加高浓度（1 mM）重金属，代表急性污染，会导致两种菌株的离子组发生相当大的变化。引人注目的是，与野生型对照相比，Tor1 缺失细胞在处理后镍含量明显降低（644.1 ± 49 与 2096.8 ± 75 μg/g），表明其在镍转运中的重要作用。总之，我们的研究结果预测了 Tor1 信号在金属摄取和/或代谢中的未知作用。
