Application of curcumin as a green and new sorbent in deep eutectic solvent-based dispersive micro-solid phase extraction of several polycyclic aromatic hydrocarbons from honey samples prior to gas chromatography-mass spectrometry determination.

A green, simple, and efficient dispersive micro-solid phase extraction method was developed for the extraction of polycyclic aromatic hydrocarbons from honey samples. In this method, for the first time, curcumin was used as an efficient and green sorbent to extract the analytes from the sample. After that the adsorbed analytes were eluted using a deep eutectic solvent prepared by mixing tetrabutylammonium chloride: ethylene glycol and analyzed by gas chromatography-mass spectrometry. Important experimental factors affecting adsorption and desorption steps of the method were optimized and under optimal experimental conditions, low limits of detection (0.14-0.37 ng/g) and quantification (0.49-1.3 ng/g), wide linear range (1.3-500 ng/g) with a coefficient of determination ≥0.994 were obtained. Relative standard deviation values for intra- and interday precisions were ≤7.5% for all of the analytes at a concentration of 2 ng/g for each analyte (n = 6). Extraction recovery of the method was in the range of 72-81%. Finally, 20 honey samples were analyzed and the analytes were successfully detected. The method is environment friendly because of the use of curcumin as a sorbent. Also, biodegradability of the used deep eutectic solvent components is another advantage of the method.