Department of Stomatology, The Children's Hospital, Zhejiang University School of Medicine, National Clinic Research Center for Child Health, No. 3333 Binsheng Road, Binjiang District, Zhejiang Province, Hangzhou, 310052, PR China.
Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou, 310058, No. 866 Yuhangtang Road, Xihu District, Zhejiang Province, PR China.
Arch Oral Biol. 2021 Nov;131:105220. doi: 10.1016/j.archoralbio.2021.105220. Epub 2021 Jul 29.
To investigate the salivary metaproteomic characteristics of the children with and without severe early childhood caries (S-ECC).
In this study, we collected unstimulated saliva samples from 34 children (age 3-4 years) with caries free (NC, dmfs (= index of decayed, missing due to caries, or filled tooth surfaces) = 0, n = 23) and with S-ECC (dmfs≥10, n = 11). Salivary proteins were extracted and reduced, and then a Liquid Chromatography/Mass Spectrometry system was used to identify proteins.
Nearly 3000 proteins were identified in this study, and about 3.5 % of the proteins originated from human while 86 % were derived from microbes. The salivary protein types in the NC group were statistically greater than those in the S-ECC group (P <0.05). Specifically, the salivary protein types derived from microbes in the NC group were significantly greater than those in the S-ECC group. Three proteins, human lactoferrin, penicillin-binding protein 1C [Burkholderia ubonensis], human alpha-defensin 1 (F28a mutant), were decreased statistically in the NC group compared to the S-ECC group (P < 0.05). Only one protein, 50S ribosomal protein L17 secreted by Haemophilus haemolyticus, was significantly increased in the NC group compared to the S-ECC group. Salivary IgA was the top highest protein in the NC group whereas human lysozyme was the top highest protein in the S-ECC group.
The differential proteins recognized in this study may be conducive for finding a caries biomarker. Understanding the metaproteomic characteristics can help us to control the caries from human origin and microbial origin.
探讨伴有和不伴有严重婴幼儿龋（S-ECC）儿童的唾液宏蛋白质组学特征。
本研究收集了 34 名年龄在 3-4 岁的儿童（无龋组[NC]，dmfs（=龋齿、因龋缺失或填充的牙面指数）=0，n=23；S-ECC 组，dmfs≥10，n=11）的非刺激性唾液样本。提取和还原唾液蛋白后，采用液相色谱/质谱联用系统鉴定蛋白质。
本研究共鉴定出近 3000 种蛋白质，其中约 3.5%的蛋白质来源于人类，86%来源于微生物。NC 组的唾液蛋白类型明显多于 S-ECC 组（P<0.05）。具体而言，NC 组来源于微生物的唾液蛋白类型明显多于 S-ECC 组。与 S-ECC 组相比，NC 组的三种蛋白质（人乳铁蛋白、青霉素结合蛋白 1C[伯克霍尔德菌乌本恩斯]、人α-防御素 1（F28a 突变体））显著减少（P<0.05）。只有一种蛋白质（由嗜血流感菌分泌的 50S 核糖体蛋白 L17）在 NC 组中明显高于 S-ECC 组。NC 组中唾液免疫球蛋白 A 是含量最高的蛋白质，而 S-ECC 组中含量最高的蛋白质是人溶菌酶。
本研究中鉴定出的差异蛋白可能有助于发现龋齿生物标志物。了解宏蛋白质组学特征有助于我们控制人类来源和微生物来源的龋齿。
