Central Laboratory for Stem Cell Research & Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500058, Telangana, India.
Department of Gynecology, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500058, Telangana, India.
Tissue Cell. 2021 Dec;73:101631. doi: 10.1016/j.tice.2021.101631. Epub 2021 Aug 25.
The reconstruction/regeneration of human bone injuries/defects represents a crucial challenge due to the lack of suitable bio/immune compatible and implantable biological grafts. The available strategies represent implications of several types of grafting materials in the form of metals, synthetic, and various kinds of biological scaffolds; however, the lack of appropriate biological components required for activating and enhancing repair mechanisms at the lesion-site limits their wider applicability.
In this study, a unique approach for generating human osteogenic implantable grafts was developed using biofabrication technology. Using a gradient change of detergents and continuous agitation, developed a unique technique to generate completely cell-free amnion and chorion scaffolds. The absence of cellular components and integrity of biological and mechanical cues within decellularized human amnion (D-HAM) and chorion (D-HCM) were evaluated and compared with fresh membranes. Allogenic bone grafts were prepared through induction of human mesenchymal stem cells (hMSCs) into osteogenic cells on D-HAM and D-HCM and evaluated for their comparative behavior at the cellular, histological and molecular levels.
The common decellularization process resulted in an efficient way to generate D-HAM and D-HCM while retaining their intact gross-anatomical architecture, surface morphology, extracellular matrix components, and mechanical properties. Both these scaffolds supported better growth of human umbilical cord blood derived MSCs as well as osteogenic differentiation. Comparative investigation revealed better growth rate and differentiation on D-HCM compared to D-HAM and control conditions.
D-HCM could be used as a better choice for producing suitable allogenic bone grafts for efficient bone healing applications.
由于缺乏合适的生物/免疫相容性和可植入生物移植物，人类骨损伤/缺陷的重建/再生是一个重大挑战。现有的策略代表了多种类型的移植物材料以金属、合成物和各种生物支架的形式的应用；然而，缺乏激活和增强病变部位修复机制所需的适当生物成分限制了它们更广泛的适用性。
在这项研究中，使用生物制造技术开发了一种生成人类成骨可植入移植物的独特方法。使用去污剂的梯度变化和连续搅拌，开发了一种独特的技术来生成完全无细胞的羊膜和胎盘支架。脱细胞人羊膜 (D-HAM) 和脱细胞人胎盘 (D-HCM) 内不存在细胞成分和生物力学线索的完整性，并与新鲜膜进行了比较。通过诱导人间充质干细胞 (hMSCs) 向 D-HAM 和 D-HCM 上的成骨细胞分化，制备同种异体骨移植物，并在细胞、组织学和分子水平上对其进行比较。
普通的脱细胞过程可以有效地生成 D-HAM 和 D-HCM，同时保留其完整的大体解剖结构、表面形态、细胞外基质成分和机械性能。这两种支架都支持人脐带血来源的间充质干细胞更好的生长和成骨分化。比较研究显示，D-HCM 比 D-HAM 和对照条件更有利于细胞生长和分化。
D-HCM 可作为生产适合同种异体骨移植物的更好选择，用于有效的骨愈合应用。
