State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P. R. China.
J Sep Sci. 2021 Nov;44(21):3914-3923. doi: 10.1002/jssc.202100293. Epub 2021 Sep 12.
An intelligent chemometric second-order calibration method called alternating trilinear decomposition- assisted multivariate curve resolution combined with high-performance liquid chromatography-diode array detection was used for the simultaneous quantification of nine tyrosine kinase inhibitors in three complex biological systems. The method allows simultaneous quantification of the components in different biological matrices without the need for cumbersome pre-treatment steps, complex elution conditions, and complete peak separation. Even with the varying time shift, severe peak overlap, and various unknown interferences, the proposed method can extract pure chromatographic and spectroscopic information for each analyte, while providing accurate qualitative and quantitative results of nine common tyrosine kinase inhibitors in three different biological matrices. All the drugs were eluted in 7 min. The results showed that the nine drugs in each matrix showed good linearity (r > 0.984) in the calibration range with a root mean square error of calibration less than 0.9 μg/mL. The average spiked recoveries of the target analytes were all in the range of 83.4-110.0%, with standard deviations less than 9.0%. Finally, the classical method was used to validate the proposed method. In comparison to the traditional method, the proposed strategy is accuracy, simultaneous, and interference-free.
一种名为交替三线分解辅助多元曲线解析结合高效液相色谱-二极管阵列检测的智能化学计量二阶校准方法被用于同时定量三种复杂生物体系中的九种酪氨酸激酶抑制剂。该方法允许在无需繁琐的预处理步骤、复杂的洗脱条件和完全的峰分离的情况下，同时定量不同生物基质中的成分。即使存在时移、严重的峰重叠和各种未知干扰，该方法也可以为每个分析物提取纯净的色谱和光谱信息，同时提供三种不同生物基质中九种常见酪氨酸激酶抑制剂的准确定性和定量结果。所有药物均在 7 分钟内洗脱。结果表明，在每个基质的校准范围内，九种药物均表现出良好的线性（r>0.984），校准的均方根误差小于 0.9μg/mL。目标分析物的平均加标回收率均在 83.4-110.0%范围内，标准偏差小于 9.0%。最后，使用经典方法验证了所提出的方法。与传统方法相比，该策略具有准确性、同时性和无干扰性。
