Arikawa Andrea Y, Samavat Hamed, Kurzer Mindy S
Department of Nutrition and Dietetics, University of North Florida, Jacksonville, FL, USA.
Department of Clinical and Preventive Nutrition Sciences, Rutgers University, Newark, NJ, USA.
J Menopausal Med. 2021 Aug;27(2):79-86. doi: 10.6118/jmm.21005.
The purpose of this cross-sectional study was to examine whether single nucleotide polymorphisms (SNPs) in enzymes that metabolize sex steroid hormones were associated with the blood levels of these hormones in postmenopausal women and if the use of menopausal hormone therapy (MHT) could modify this association.
Baseline data were collected from 932 postmenopausal women enrolled in the Minnesota Green Tea Trial. Participants filled out a questionnaire about their demographics, lifestyle factors, and medical and reproductive history. Free, bioavailable, and total serum levels of reproductive hormones were measured through liquid chromatography/tandem mass spectrometry. For genotyping of (rs10928303), (rs10929301, rs11673726), (rs1105879, rs2070959, rs6759892), (rs10167119), (rs7439366), and (rs9282861, rs1968752), mass spectrometry based on multiplex methods and TaqMan assays were performed. Adjusted linear models were fit to assess the associations between SNPs and blood hormones using age, body mass index (BMI), and MHT as covariates.
The mean age was 59.8 years, and the mean BMI was 25.1 kg/m². Past or recent use of MHT was reported by 41.2% of the participants. SNPs in (rs1968752 and rs9282861) and (rs11673726) genes were significantly associated with estrone levels, whereas SNPs in (rs6759892) and (rs10167119) genes were significantly associated with bioavailable estradiol levels.
There was no evidence that MHT use modified the association between SNPs and sex-steroid hormone levels; however, further studies are needed to establish the potential clinical significance of (rs11673726), (rs6759892), and (rs10167119) SNPs and the modulation of hormone levels in postmenopausal women.
本横断面研究旨在探讨代谢性激素的酶中的单核苷酸多态性（SNP）是否与绝经后女性这些激素的血液水平相关，以及绝经激素治疗（MHT）的使用是否会改变这种关联。
收集了参加明尼苏达绿茶试验的932名绝经后女性的基线数据。参与者填写了一份关于其人口统计学、生活方式因素以及医疗和生殖史的问卷。通过液相色谱/串联质谱法测量生殖激素的游离、生物可利用和总血清水平。对于（rs10928303）、（rs10929301、rs11673726）、（rs1105879、rs2070959、rs6759892）、（rs10167119）、（rs7439366）和（rs9282861、rs1968752）的基因分型，采用基于多重方法的质谱分析和TaqMan分析。使用年龄、体重指数（BMI）和MHT作为协变量，拟合调整后的线性模型以评估SNP与血液激素之间的关联。
平均年龄为59.8岁，平均BMI为25.1kg/m²。41.2%的参与者报告了过去或近期使用MHT的情况。（rs1968752和rs9282861）基因以及（rs11673726）基因中的SNP与雌酮水平显著相关，而（rs6759892）基因和（rs10167119）基因中的SNP与生物可利用雌二醇水平显著相关。
没有证据表明使用MHT会改变SNP与性类固醇激素水平之间的关联；然而，需要进一步研究以确定（rs11673726）、（rs6759892）和（rs10167119）SNP在绝经后女性中对激素水平调节的潜在临床意义。
