Dekleva M L, Strohl W R
Department of Microbiology, Ohio State University, Columbus 43210.
Can J Microbiol. 1987 Dec;33(12):1129-32. doi: 10.1139/m87-198.
Cultures of Streptomyces peucetius often were observed to divert spontaneously from the production of anthracycline antibiotics to the excretion of acidic metabolites, whether grown in a defined medium or a nutrient-rich medium. The predominant excreted acidic metabolites were determined by high-performance liquid chromatography to be pyruvate and 2-oxoglutarate. The acidification occurred at high frequencies when glucose was the primary carbon source in a defined medium, at intermediate frequencies when fructose was the primary carbon source, and did not occur when either maltose, starch, or dextrin were used as primary carbon sources.
无论在限定培养基还是富营养培养基中培养，常常观察到变铅青链霉菌的培养物会自发地从蒽环类抗生素的产生转向酸性代谢产物的排泄。通过高效液相色谱法测定，主要排泄的酸性代谢产物是丙酮酸和2-氧代戊二酸。当葡萄糖是限定培养基中的主要碳源时，酸化以高频率发生；当果糖是主要碳源时，酸化以中等频率发生；而当麦芽糖、淀粉或糊精用作主要碳源时，则不会发生酸化。
