Barman S A, Olson M D, Saari J T
Department of Physiology, University of North Dakota School of Medicine, Grand Forks 58202.
Cardiovasc Res. 1987 Aug;21(8):576-81. doi: 10.1093/cvr/21.8.576.
To determine whether histamine's effect on microvascular permeability might be associated with altered calcium binding, the influence of histamine on lanthanum binding to vascular endothelium was assessed in the presence of verapamil. Isolated rabbit hearts were perfused firstly with histamine, secondly with histamine and verapamil, and subsequently with histamine in the presence of lanthanum and verapamil. Examination by transmission electron microscopy showed that 92% of microvessels examined contained bound lanthanum on plasma membranes, plasmalemmal vesicles, or endothelial clefts of vascular endothelium, or all three, in both atria and ventricles, which is in contrast to the low proportion (4%) of microvessels containing bound lanthanum found in the presence of verapamil alone. The enhancement of lanthanum binding was reduced by both H1 and H2 receptor antagonists. This evidence supports the hypothesis that the increase in vascular permeability with histamine may be associated with increased calcium binding to vascular endothelial membranes and that both H1 and H2 receptors play a part in this activity.
为了确定组胺对微血管通透性的影响是否可能与钙结合改变有关，在维拉帕米存在的情况下评估了组胺对镧结合到血管内皮的影响。分离的兔心脏首先用组胺灌注，其次用组胺和维拉帕米灌注，随后在镧和维拉帕米存在的情况下用组胺灌注。透射电子显微镜检查显示，在心房和心室中，所检查的微血管中有92%在质膜、质膜小泡或血管内皮的内皮裂隙上含有结合的镧，或三者都有，这与仅在维拉帕米存在时发现的含有结合镧的微血管的低比例（4%）形成对比。H1和H2受体拮抗剂均降低了镧结合的增强。这一证据支持了这样的假设，即组胺引起的血管通透性增加可能与钙结合到血管内皮膜增加有关，并且H1和H2受体在这一活动中都起作用。
