Yan Fen, Cai Ting, Wu Yunyun, Chen Shuqiong, Chen Junying
College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China.
College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China.
Genomics. 2021 Nov;113(6):3750-3761. doi: 10.1016/j.ygeno.2021.08.017. Epub 2021 Aug 28.
This study aimed to explore the effects of recombinant serine protease treatment on the development of post-harvest loquat diseases, fruit quality, and disease resistance enzyme activities. It also sought to analyze differential genes expression using RNA-seq technology. Transcriptomics analysis revealed 708 and 398 differentially expressed genes (DEGs) in loquat fruits treated with serine protease for 24 and 48 h. Furthermore, 2198 DEGs were obtained between 24 and 48 h after treatment. The genes encoding JAZ, MYC2 and ERF in the plant signal transduction pathway were significantly up-regulated. The resistance-related genes, such as PPO, PAL, TLP, WRKY, and transcription factors were also significantly up-regulated. These results indicated that the recombinant serine protease can induce plant signal transduction pathway in loquat fruit. The expression of some resistance-related genes enhanced the disease resistance of loquat fruit and revealed the molecular mechanism of loquat fruit resistance induced by recombinant serine protease.
本研究旨在探讨重组丝氨酸蛋白酶处理对采后枇杷病害发生、果实品质及抗病酶活性的影响。同时，利用RNA测序技术分析差异基因表达。转录组学分析显示，用丝氨酸蛋白酶处理24小时和48小时的枇杷果实中分别有708个和398个差异表达基因（DEG）。此外，处理后24小时至48小时之间获得了2198个DEG。植物信号转导途径中编码JAZ、MYC2和ERF的基因显著上调。与抗性相关的基因，如PPO、PAL、TLP、WRKY以及转录因子也显著上调。这些结果表明，重组丝氨酸蛋白酶可诱导枇杷果实中的植物信号转导途径。一些抗性相关基因的表达增强了枇杷果实的抗病性，并揭示了重组丝氨酸蛋白酶诱导枇杷果实抗性的分子机制。
