Ran Zhi-Fang, Yang Xiao-Tong, Li Rui, Zhou Jie, Zhang Yong-Qing, Guo Lan-Ping
School of Biological Science and Technology, University of Ji'nan Ji'nan 250022, China School of Pharmacy, Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
School of Biological Science and Technology, University of Ji'nan Ji'nan 250022, China.
Zhongguo Zhong Yao Za Zhi. 2021 Aug;46(16):4103-4110. doi: 10.19540/j.cnki.cjcmm.20210523.107.
In this study, the colonization, diversity and relative abundance of arbuscular mycorrhizal fungi(AMF) in the roots of Panax quinquefolius in different habitats of Shandong province were analyzed by staining-microscopy and high-throughput sequencing. The data were analyzed by bioinformatics tools and statistical software. The results showed that the roots of P. quinquefolius in different habitats were colonized by AMF with different rates and intensities. The AMF in roots of P. quinquefolius belong to three genera, three families, three orders, one class and one phylum. At the level of order, the AMF mainly included Paraglomerales(52.48%), Glomerales(25.60%) and Archaeosporales(3.08%). At the level of family, the AMF were dominated by Paraglomeraceae(52.48%), Glomeraceae(18.94%) and Claroideoglomeraceae(3.05%). At the level of genus, Paraglomus(51.46%), Glomus(20.01%) and Claroideoglomus(3.52%) accounted for a large proportion, of which Paraglomus and Glomus were dominant. Cluster analysis showed that the AMF in roots of P. quinquefolius with close geographical locations could be clustered together. In this study, the diversity and dominant germplasm resources of AMF in roots of P. quinquefolius cultivated in the main producing areas were identified, which provi-ded basic data for revealing the quality formation mechanism of P. quinquefolius medicinal materials from the perspective of environment.
本研究采用染色显微镜观察和高通量测序技术,分析了山东省不同生境下西洋参根内丛枝菌根真菌(AMF)的定殖情况、多样性及相对丰度。利用生物信息学工具和统计软件对数据进行分析。结果表明,不同生境下西洋参的根均被不同比例和强度的AMF定殖。西洋参根内的AMF隶属于1个门、1个纲、3个目、3个科、3个属。在目水平上,AMF主要包括类球囊霉目(52.48%)、球囊霉目(25.60%)和原囊霉目(3.08%)。在科水平上,以类球囊霉科(52.48%)、球囊霉科(18.94%)和盾巨孢囊霉科(3.05%)为主。在属水平上,类球囊霉属(51.46%)、球囊霉属(20.01%)和盾巨孢囊霉属(3.52%)占比较大,其中类球囊霉属和球囊霉属为优势属。聚类分析表明,地理位置相近的西洋参根内AMF可聚为一类。本研究明确了主产区栽培西洋参根内AMF的多样性及优势种质资源,为从环境角度揭示西洋参药材质量形成机制提供了基础数据。