以乙醇为电子供体，利用简单基质和废活性污泥生产中链脂肪酸。
Medium chain fatty acids production from simple substrate and waste activated sludge with ethanol as the electron donor.
机构信息
School of Civil Engineering, Guangzhou University, Guangzhou, 510006, China; State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China.
College of Environment and Ecology, Chongqing University, Chongqing, 400044, China; State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China.
出版信息
Chemosphere. 2021 Nov;283:131278. doi: 10.1016/j.chemosphere.2021.131278. Epub 2021 Jun 19.
Production of MCFAs (Medium-chain fatty acids) from simple substrate (i.e., ethanol and acetate) and WAS with chain elongation microbiome was investigated in this study. The results showed that rapid production of MCFAs was observed when simple substrate was utilized. 1889 mg/L of caproate and 3434 mg/L of butyrate were achieved after 10 d's reaction. H proportion in the headspace could reach as high as 10.1% on day 8 and then declined quickly. However, when WAS was used, the bacterial consortia was not able to hydrolyze WAS efficiently, which resulted in poor MCFAs production performance. Presence of ethanol could improve the hydrolysis process to a limited degree, which resulted in solubilization of a small fraction of protein and carbohydrate. Around 33.8% and 36.9% of the total detected electrons on day 6 in the 50 mM and 100 mM tests were extracted from WAS respectively. Those results indicate that the chain elongation microbial consortia tended to receive electrons form ethanol directly other than the complex WAS.
本研究考察了利用简单底物（即乙醇和乙酸盐）和具有链延长微生物组的 WAS 生产 MCFAs（中链脂肪酸）的情况。结果表明，利用简单底物时可观察到 MCFAs 的快速生产。反应 10 天后，可获得 1889mg/L 的己酸和 3434mg/L 的丁酸。在第 8 天，顶空 H 比例高达 10.1%，然后迅速下降。然而，当使用 WAS 时，细菌群落不能有效地水解 WAS，导致 MCFAs 生产性能不佳。乙醇的存在可以在一定程度上改善水解过程，导致一小部分蛋白质和碳水化合物溶解。在 50mM 和 100mM 试验中，第 6 天总检测到的电子中约有 33.8%和 36.9%分别来自 WAS。这些结果表明，链延长微生物群落倾向于直接从乙醇而不是复杂的 WAS 中获取电子。
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