Petersen J S, Christensen S
Department of Pharmacology, University of Copenhagen, Denmark.
Ren Physiol. 1987;10(2):102-9. doi: 10.1159/000173119.
The renal clearances (C) of p-aminohippurate (PAH) and tetraethylammonium (TEA) as markers for renal plasma flow (RPF) were compared in rats under different experimental conditions. CTEA and CPAH were of the same magnitude and varied synchronously during volume expansion and anesthesia. The renal extractions of the two substances were closely correlated and averaged 85.9% for PAH and 88.7% for TEA (p less than 0.01). High doses of furosemide (120 mg/kg as intravenous bolus) reversibly decreased the CPAH/CTEA ratio, suggesting that furosemide may compete for PAH secretion in the proximal tubule. A similar effect was observed after administration of probenecid. The results indicate that TEA is a more reliable indicator of RPF than PAH in studies involving accumulation of endogenous or exogenous organic anions.
在不同实验条件下，比较了用对氨基马尿酸（PAH）和四乙铵（TEA）作为肾血浆流量（RPF）标志物时大鼠的肾清除率（C）。在容量扩张和麻醉期间，CTEA和CPAH大小相同且同步变化。两种物质的肾摄取密切相关，PAH平均为85.9%，TEA平均为88.7%（p<0.01）。高剂量呋塞米（静脉推注120mg/kg）可逆性降低CPAH/CTEA比值，提示呋塞米可能在近端小管竞争PAH分泌。给予丙磺舒后观察到类似效应。结果表明，在涉及内源性或外源性有机阴离子蓄积的研究中，TEA是比PAH更可靠的RPF指标。
