Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, 920-1192, Japan.
Chemistry. 2021 Oct 21;27(59):14582-14585. doi: 10.1002/chem.202102333. Epub 2021 Sep 30.
Herein is reported a circularly polarized luminescent (CPL) probe that can respond to the chirality of nucleic acids. An achiral nanostructure was prepared by the hybridization of symmetric serinol nucleic acid (SNA) containing pyrene-modified residues. When chiral oligomers that were complementary to the SNA were added, they induced helicity into the SNA nanowire. Efficient circular dichroism (CD) signal amplification was observed when pyrene was attached to uracil bases through a rigid alkynyl linker. Both CPL and CD signals were observed; they depended on the chirality of the added acyclic threoninol nucleic acid (aTNA) oligomer. This system can be used to convert the chirality of chiral biomolecules into chiroptical signals.
本文报道了一种可响应核酸手性的圆偏振发光（CPL）探针。通过含有芘修饰残基的对称丝氨酸核酸（SNA）的杂交制备了非手性纳米结构。当添加与 SNA 互补的手性寡聚物时，它们会诱导 SNA 纳米线形成螺旋结构。当芘通过刚性炔基连接子连接到尿嘧啶碱基时，观察到有效的圆二色性（CD）信号放大。同时观察到 CPL 和 CD 信号，它们取决于添加的非环苏氨酸核酸（aTNA）寡聚物的手性。该系统可用于将手性生物分子的手性转化为手性光学信号。
