[经皮耳迷走神经刺激对功能性消化不良大鼠自主神经功能的影响]

[Effects of transcutaneous auricular vagus nerve stimulation on autonomic nervous function in rats with functional dyspepsia].

作者信息

Hou Li-Wei, Rong Pei-Jing, Li Liang, Wei Wei, Fang Ji-Liang, Zhang Jin-Ling, Wang Jun-Ying

机构信息

Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102.

出版信息

Zhen Ci Yan Jiu. 2021 Aug 25;46(8):663-70. doi: 10.13702/j.1000-0607.200965.

DOI:10.13702/j.1000-0607.200965

PMID:34472751

Abstract

OBJECTIVE

To observe the effect of transcutaneous auricular vagus nerve stimulation (taVNS) on the autonomic nerve function in a rat model of functional dyspepsia (FD), so as to explore the mechanism of taVNS underlying regulation of FD.

METHODS

SD rats were randomly divided into normal control group(n=8) and FD model group(n=26).The FD model was replicated with iodoacetamide gavage. The FD model rats were randomly divided into model, taVNS, sham-taVNS and Zusanli(ST36) groups, with 6 rats in each group. Rats in the taVNS group received electrical stimulation of auricular concha,while the sham-taVNS group received no electrical stimulation and rats in the ST36 group received stimulation at ST36 for 30 min once daily for 14 consecutive days. Cervical trapezius electromyography score and abdominal withdrawal reflex (AWR) score were used to evaluate gastric sensitivity. Histopathological changes of the gastric antrum tissue were observed under microscope after H.E. staining. Autonomic nerve function in rats was recorded and assessed by heart rate variability(HRV). The content of acetylcholine (Ach) and the expression of Ach receptor M3R in gastric antrum was detect by ELISA and Western blot, separately.

RESULTS

Compared with the normal control group, the cervical trapezius electromyography and AWR scores of the model group increased (<0.01, <0.001), and there was no erosion in the gastric antral mucosa and muscle layer. The high-frequency power (HF) in HRV decreased (<0.05), the ratio of low-frequency power/high-frequency power (LF/HF) increased (<0.001), and the Ach content and its receptor M3R expression in gastric antrum tissue decreased (<0.05). Following interventions, the cervical trapezius electromyography and AWR scores decreased (<0.01,<0.001, <0.05), HF in HRV increased and LF/HF decreased(<0.01，<0.05,<0.001), and the content of Ach in gastric antrum tissue and the expression of its receptor M3R increased (<0.01, <0.05) in both taVNS and ST36 groups relevant to the model group.

CONCLUSION

taVNS can increase the activity of the vagus nerve and regulate the balance of the autonomic nerve function, which may be one of the mechanisms of taVNS in reducing the gastric sensitivity of rats with FD. In regulating the vagus nerve function, taVNS and acupuncture at ST36 acupoint have the similar effects.

摘要

目的

观察经皮耳迷走神经刺激（taVNS）对功能性消化不良（FD）大鼠模型自主神经功能的影响，以探讨taVNS调节FD的机制。

方法

将SD大鼠随机分为正常对照组（n = 8）和FD模型组（n = 26）。采用碘乙酰胺灌胃复制FD模型。将FD模型大鼠随机分为模型组、taVNS组、假taVNS组和足三里（ST36）组，每组6只。taVNS组大鼠接受耳甲电刺激，假taVNS组不接受电刺激，ST36组大鼠于ST36穴接受刺激，每天1次，每次30分钟，连续14天。采用颈阔肌肌电图评分和腹部回撤反射（AWR）评分评估胃敏感性。苏木精-伊红（H.E.）染色后在显微镜下观察胃窦组织的组织病理学变化。通过心率变异性（HRV）记录和评估大鼠的自主神经功能。分别采用酶联免疫吸附测定（ELISA）和蛋白质免疫印迹法（Western blot）检测胃窦中乙酰胆碱（Ach）含量及Ach受体M3R的表达。

结果

与正常对照组相比，模型组颈阔肌肌电图和AWR评分升高（<0.01，<0.001），胃窦黏膜及肌层无糜烂。HRV中的高频功率（HF）降低（<0.05），低频功率/高频功率比值（LF/HF）升高（<0.001），胃窦组织中Ach含量及其受体M3R表达降低（<0.05）。干预后，taVNS组和ST36组与模型组相比，颈阔肌肌电图和AWR评分降低（<0.01，<0.001，<0.05），HRV中的HF升高，LF/HF降低（<0.01，<0.05，<0.001），胃窦组织中Ach含量及其受体M3R表达升高（<0.01，<0.05）。