Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
J Immunol Methods. 2021 Nov;498:113135. doi: 10.1016/j.jim.2021.113135. Epub 2021 Aug 31.
In recent years, there has been an expansion in the use of flow cytometry (FC) immunophenotyping in the diagnosis and monitoring of childhood solid neoplasms. Neuroblastoma (NB), in turn, is the most common extracranial solid tumor in childhood. In the present study, we sought to compare FC and anatomopathological examination (PA) / immunohistochemistry (IHC) of children diagnosed or suspected with NB. The median age was 59 months (minimum 0; maximum 325 months), of these 12 were male (57.1%, 12/21). Forty-eight samples (27 bone marrow (BM), 10 peripheral blood (PB), 8 primary tumors (PT) and 2 liver nodules (HN) and 1 rib fragment (RF)) from 21 patients were evaluated. Twenty-nine samples were from patients with clinical suspicion while 19 samples were from patients with previously confirmed diagnosis. Thirteen samples (7 BM, 5 PT and 1 HN) presented NB when analyzed in FC while 8 (3 BM and 5 PT) samples were positive for NB in the PA/IHC. They were concordant in 88.9% of the cases. No NB cells were identified in any PB. Considering the PA as the gold standard, the FC obtained a sensitivity of 100%, a specificity of 86%, a positive predictive value of 67% and a negative predictive value of 100%. This study demonstrates that FC can be used as a methodology for diagnosis and assessment of NB involvement. In addition, FC has the advantage of allowing a quick diagnosis and accurate classification of the disease, and can also assist in monitoring the treatment.
近年来，流式细胞术（FC）免疫表型分析在儿童实体瘤的诊断和监测中的应用不断扩大。神经母细胞瘤（NB）是儿童期最常见的颅外实体瘤。本研究旨在比较 FC 与解剖病理检查（PA）/免疫组织化学（IHC）在诊断或疑似 NB 儿童中的应用。中位年龄为 59 个月（最小 0；最大 325 个月），其中男性 12 例（57.1%，12/21）。对 21 例患者的 48 份样本（27 份骨髓（BM）、10 份外周血（PB）、8 份原发肿瘤（PT）、2 份肝结节（HN）和 1 份肋骨碎片（RF））进行了评估。29 份样本来自临床疑似患者，19 份样本来自已确诊患者。在 FC 分析中，13 份（7 份 BM、5 份 PT 和 1 份 HN）样本显示为 NB，而 8 份（3 份 BM 和 5 份 PT）样本在 PA/IHC 中呈 NB 阳性。这些结果在 88.9%的病例中是一致的。任何 PB 中均未发现 NB 细胞。以 PA 为金标准，FC 的敏感性为 100%，特异性为 86%，阳性预测值为 67%，阴性预测值为 100%。本研究表明，FC 可作为 NB 诊断和评估的一种方法。此外，FC 具有快速诊断和准确分类疾病的优势，还可辅助监测治疗。
