State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming, China.
Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Pest Manag Sci. 2022 Jan;78(1):274-286. doi: 10.1002/ps.6630. Epub 2021 Sep 19.
Elevated CO can directly affect the toxicity of insecticides to insects and the physiological response of insects to insecticides. Frankliniella occidentalis and F. intonsa are highly destructive pests that target horticultural crops. Spinetoram is an effective pesticide against thrips. This study sought to explore the effect of elevated CO on efficacy of spinetoram against F. occidentalis and F. intonsa and effect of the spinetoram on activities of protective and detoxifying enzymes under elevated CO . Notably, these enzymes can be exploited in further studies to develop interventions for thrips resistance management.
Toxicity bioassay showed that the LC values of F. occidentalis and F. intonsa exposed to spinetoram at elevated CO (800 μL L concentration) for 48 h was 0.08 and 0.006 mg L , respectively, which is 0.62 and 0.75 times of the values at ambient CO (400 μL L concentration). The findings showed that elevated CO decreased activities of the superoxide dismutase and acetylcholinesterase in thrips, while increasing the activities of carboxylesterase and glutathione S-transferase. However, spinetoram increased activities of protective and detoxifying enzymes in both thrips under the two CO levels. Elevated CO and spinetoram affect the physiological enzyme activity in thrips synergistically, and the activities of analyzed enzymes were generally higher in F. occidentalis than in F. intonsa.
Elevated CO amplifies the efficacy of spinetoram on thrips, F. intonsa is more susceptibility to spinetoram than F. occidentalis and the latter showed better adaptation to adverse conditions than the former. © 2021 Society of Chemical Industry.
升高的 CO 可以直接影响杀虫剂对昆虫的毒性和昆虫对杀虫剂的生理反应。西方花蓟马和烟蓟马是对园艺作物具有高度破坏性的害虫。螺虫乙酯是一种防治蓟马的有效农药。本研究旨在探讨升高的 CO 对螺虫乙酯防治西方花蓟马和烟蓟马效果的影响，以及在升高的 CO 下螺虫乙酯对保护和解毒酶活性的影响。值得注意的是，这些酶可以在进一步的研究中被利用，以开发防治蓟马抗药性的干预措施。
毒性生物测定表明，在升高的 CO（800 μL·L 浓度）下暴露于螺虫乙酯 48 小时后，西方花蓟马和烟蓟马的 LC 值分别为 0.08 和 0.006 mg·L，分别是在环境 CO（400 μL·L 浓度）下的 0.62 倍和 0.75 倍。研究结果表明，升高的 CO 降低了蓟马中超氧化物歧化酶和乙酰胆碱酯酶的活性，而增加了羧酸酯酶和谷胱甘肽 S-转移酶的活性。然而，螺虫乙酯在两种 CO 水平下均增加了两种蓟马的保护和解毒酶的活性。升高的 CO 和螺虫乙酯协同影响蓟马的生理酶活性，且在西方花蓟马中分析的酶活性普遍高于烟蓟马。
升高的 CO 放大了螺虫乙酯对蓟马的防治效果，烟蓟马比西方花蓟马对螺虫乙酯更敏感，后者比前者更能适应不利条件。© 2021 化学工业协会。
