Setouchi Parasite Biodiversity Laboratory, 3-2-20-103, Ushita-Honmachi, Higashi-ku, Hiroshima 732-0066, Japan; Laboratory of Bioresources, National Institute for Basic Biology, 38, Nishigonaka, Myodaiji, Okazaki, Aichi 444-8787, Japan.
Parasitol Int. 2021 Dec;85:102448. doi: 10.1016/j.parint.2021.102448. Epub 2021 Sep 1.
Molecular studies of the Capsalidae suggested that the genus Benedenia is polyphyletic, but a taxonomic organization of the genus that reflects molecular data has not yet been proposed. As a result of molecular analysis (28S rDNA, ITS1-5.8S-ITS2, and cox1 data) including specimens of Benedeniinae newly obtained from Okinawa-jima Island in Japan, two new genera and the revival of Tareenia independent to the genus Benedenia are proposed. Gracilobenedenia n. gen. is distinguished from the other genera of Benedeniinae based on morphological characteristics. This new genus comprises 6 species: G. lutjani n. comb. (type species), G. anticavaginata n. comb., G. rohdei n. comb., G. beverleyburtonae n. comb., G. kuremibai n. gen., n. sp., and G. hichi n. gen., n. sp. Armatobenedenia n. gen. is a monotypic genus for A. armata n. comb. The present molecular phylogenetic analysis showed the independence of Tareenia, and it can be morphologically separated from the other benedeniines. Four species including two new species obtained from Okinawa-jima Island are reported: G. kuremibai n. sp., G. hichi n. sp., G. lutjani n. comb., and Metabenedeniella parva. Furthermore, in the species identification and phylogenetic analysis of capsalids, the usefulness of not only the 28S rDNA but also ITS and the cox1 regions was suggested. These genes were evaluated the efficacy of those regions in DNA barcoding, and the ITS and cox1 regions shown to be useful for DNA barcoding in capsalids compared to the 28S rDNA sequence.
对胶囊科的分子研究表明，本尼登属是多系的，但尚未提出反映分子数据的本尼登属分类组织。由于包括从日本冲绳岛新获得的贝登尼inae 标本的分子分析（28S rDNA、ITS1-5.8S-ITS2 和 cox1 数据），提出了两个新属和独立于本尼登属的塔雷尼娅的复兴。 Gracilobenedenia n. gen. 基于形态特征与其他贝登尼inae 属区别开来。这个新属包括 6 个种：G. lutjani n. comb.（模式种）、G. anticavaginata n. comb.、G. rohdei n. comb.、G. beverleyburtonae n. comb.、G. kuremibai n. gen.，n. sp. 和 G. hichi n. gen.，n. sp.。Armatobenedenia n. gen. 是 A. armata n. comb. 的单型属。目前的分子系统发育分析表明，塔雷尼娅是独立的，可以与其他贝登尼inae 形态上区分开来。从冲绳岛获得的四个物种包括两个新种：G. kuremibai n. sp.、G. hichi n. sp.、G. lutjani n. comb. 和 Metabenedeniella parva。此外，在胶囊的物种鉴定和系统发育分析中，不仅 28S rDNA，而且 ITS 和 cox1 区域都很有用。这些基因评估了这些区域在 DNA 条形码中的功效，与 28S rDNA 序列相比，ITS 和 cox1 区域在胶囊的 DNA 条形码中显示出有用性。
