多重侧向流动免疫层析测定法是一种有效的检测碳青霉烯酶的方法，不会有 OXA-48 样交叉反应的风险。
Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity.
机构信息
National Institute for Antibiotic Resistance and Infection Control, Ministry of Health, 6 Weizmann St., 6423906, Tel-Aviv, Israel.
Clinical Microbiology Laboratory, Tel-Aviv Sourasky Medical Center, Tel-Aviv, Israel.
出版信息
Ann Clin Microbiol Antimicrob. 2021 Sep 4;20(1):61. doi: 10.1186/s12941-021-00469-0.
Abstract
BACKGROUND
It is essential to detect carriers of carbapenemase-producing Enterobacterales in order to implement infection control measures. The objectives of this study was to evaluate the NG-Test CARBA 5 (CARBA 5) assay for detection of five carbapenemases and to assess the cross reactivity of other OXA-type carbapenemases with the OXA-48-like specific antibodies.
METHODS
A total of 197 Enterobacterales isolates were tested. To evaluate the cross reactivity, 73 carbapenem-resistant A. baumannii, harboring OXA-type variants, were tested. Polymerase chain reaction (PCR) served as gold standard for carbapenemase identification.
RESULTS
Excellent agreement was found between PCR and CARBA 5, for all but one isolate. The single false positive result (a bla positive S. marcescens isolate) was incorrectly positive for bla by CARBA 5. No cross reactivity was observed. The sensitivity and specificity were 100.0% and 98.0%, respectively.
CONCLUSIONS
The CARBA 5 assay is highly sensitive and specific and is recommended as a tool for the detection of the main carbapenemases of interest in clinical microbiology laboratories.
摘要
背景
检测产碳青霉烯酶肠杆菌科（Carbapenemase-producing Enterobacterales，简称 CPE）携带者对于实施感染控制措施至关重要。本研究旨在评估 NG-Test CARBA 5（CARBA 5）检测五种碳青霉烯酶的能力，并评估其他 OXA 型碳青霉烯酶与 OXA-48 样特异性抗体的交叉反应性。
方法
共检测了 197 株肠杆菌科分离株。为了评估交叉反应性，检测了 73 株携带 OXA 型变体的耐碳青霉烯类鲍曼不动杆菌。聚合酶链反应（PCR）作为碳青霉烯酶鉴定的金标准。
结果
除了一个分离株外，PCR 和 CARBA 5 之间的一致性非常好。唯一的假阳性结果（一株 bla 阳性的粘质沙雷氏菌分离株）被 CARBA 5 错误地鉴定为 bla。未观察到交叉反应。该方法的敏感性和特异性分别为 100.0%和 98.0%。
结论
CARBA 5 检测法具有高度的敏感性和特异性，推荐作为临床微生物学实验室检测主要碳青霉烯酶的工具。
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