Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, USA; OptiEnz Sensors LLC, Fort Collins, CO, USA.
Curr Opin Biotechnol. 2021 Oct;71:225-230. doi: 10.1016/j.copbio.2021.08.006. Epub 2021 Sep 2.
Precise control over bioreactor operation is desired for optimal productivity and product quality, and there is an increased drive to automation in biomanufacturing. All of these goals require sensors, not only of the basic parameters of temperature, pH, and dissolved oxygen, but of the biomass and substrate concentrations, which directly determine the outcome of the bioprocess. While there are many innovative sensing concepts for biomass and substrate concentrations, this review focuses on sensors that are in-line with the bioreactor, providing data continuously without the removal of sample from the system. The discussion emphasizes the requirements of industry for these sensors, including performance, ease of use, and cost. As the bioeconomy grows, advances in sensing technologies will be needed to achieve the automation of the future for a wider array of bioreactors.
需要对生物反应器的操作进行精确控制，以实现最佳的生产力和产品质量，并且在生物制造中越来越需要自动化。所有这些目标都需要传感器，不仅需要测量温度、pH 值和溶解氧等基本参数，还需要测量生物量和底物浓度，这些参数直接决定了生物过程的结果。虽然有许多创新的生物量和底物浓度感测概念，但本综述重点介绍与生物反应器集成的传感器，这些传感器可以连续提供数据，而无需从系统中取出样品。讨论强调了这些传感器对行业的要求，包括性能、易用性和成本。随着生物经济的发展，需要先进的感测技术来实现更广泛的生物反应器的未来自动化。
