血清巨噬细胞移动抑制因子在慢性鼻-鼻窦炎伴鼻息肉表型术前预测中的作用。
The role of serum macrophage migration inhibitory factor in preoperative prediction of chronic rhinosinusitis with nasal polyps endotypes.
机构信息
Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, Hunan, China; Hunan Province Key Laboratory of Otolryngology Critical Diseases, Changsha, Hunan, China.
Department of Otolaryngology Head and Neck Surgery, Changsha Central Hospital, University of South China, Changsha, Hunan, China.
出版信息
Int Immunopharmacol. 2021 Nov;100:108084. doi: 10.1016/j.intimp.2021.108084. Epub 2021 Sep 3.
BACKGROUND
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a highly heterogeneous disease and can be categorized into eosinophilic CRSwNP (eCRSwNP) and non-eosinophilic CRSwNP (neCRSwNP). Exploring effective biomarkers to distinguish endotypes is important for personalized therapies. The present study aims to evaluate the predictive value of serum MIF in CRSwNP endotypes.
METHODS
One hundred and twenty CRSwNP patients, including 51 eCRSwNP and 69 neCRSwNP, 40 chronic rhinosinusitis without nasal polyps (CRSsNP) patients and 40 healthy controls (HC) were enrolled. Serum MIF levels were determined by enzyme-linked immunosorbent assay (ELISA). The patients' clinical variables were analyzed for correlations with serum MIF. Receiver operating characteristic (ROC) curve and multivariate analysis were utilized to assess the predictive value of serum MIF in CRSwNP endotypes.
RESULTS
The serum MIF levels were significantly higher in CRSwNP group than CRSsNP group and HC group (P < 0.001), and the serum MIF levels were increased in eCRSwNP compared to neCRSwNP group (P = 0.006). Elevated serum MIF levels were significantly correlated with blood eosinophil (B-EOS) count (r = 0.411, P < 0.001), B-EOS percentage (r = 0.377, P < 0.001), visual analog scale score (r = 0.204, P = 0.025), tissue eosinophil (T-EOS) count (r = 0.705, P < 0.001) and T-EOS percentage (r = 0.671, P < 0.001) in CRSwNP patients. ROC curve demonstrated that serum MIF exhibited good preoperative prediction in CRSwNP endotypes (area under the curve = 0.925, P < 0.001). Multivariate regression analysis showed that serum MIF was an independent factor associated with CRSwNP endotypes.
CONCLUSIONS
This was the first study identifying serum MIF as a possible specific biomarker for preoperatively distinguishing CRSwNP endotypes. Furthermore, the serum MIF levels were found to be closely associated with the degree of mucosal eosinophil infiltration.
背景
慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）是一种高度异质性疾病，可以分为嗜酸性 CRSwNP（eCRSwNP）和非嗜酸性 CRSwNP（neCRSwNP）。探索有效的生物标志物来区分表型对于个性化治疗很重要。本研究旨在评估血清 MIF 在 CRSwNP 表型中的预测价值。
方法
纳入 120 例 CRSwNP 患者，包括 51 例 eCRSwNP 和 69 例 neCRSwNP，40 例慢性鼻-鼻窦炎不伴鼻息肉（CRSsNP）患者和 40 名健康对照者（HC）。采用酶联免疫吸附试验（ELISA）测定血清 MIF 水平。分析患者的临床变量与血清 MIF 的相关性。利用受试者工作特征（ROC）曲线和多变量分析评估血清 MIF 在 CRSwNP 表型中的预测价值。
结果
CRSwNP 组患者血清 MIF 水平明显高于 CRSsNP 组和 HC 组（P<0.001），eCRSwNP 组患者血清 MIF 水平高于 neCRSwNP 组（P=0.006）。升高的血清 MIF 水平与血嗜酸性粒细胞（B-EOS）计数（r=0.411，P<0.001）、B-EOS 百分比（r=0.377，P<0.001）、视觉模拟评分（r=0.204，P=0.025）、组织嗜酸性粒细胞（T-EOS）计数（r=0.705，P<0.001）和 T-EOS 百分比（r=0.671，P<0.001）显著相关。ROC 曲线显示，血清 MIF 对 CRSwNP 表型具有良好的术前预测能力（曲线下面积=0.925，P<0.001）。多变量回归分析表明，血清 MIF 是与 CRSwNP 表型相关的独立因素。
结论
本研究首次发现血清 MIF 可能是术前区分 CRSwNP 表型的特异性生物标志物。此外，血清 MIF 水平与黏膜嗜酸性粒细胞浸润程度密切相关。
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