Lyu Zhimai, Huang Dandan, Xie Dingyi, Chen Yanjun, Wu Chunmei, Chen Rixin, Luo Weifeng
Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China.
Department of Neurology, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, Jiangxi Province, China.
Evid Based Complement Alternat Med. 2021 Aug 26;2021:6463688. doi: 10.1155/2021/6463688. eCollection 2021.
Our previous studies demonstrated that effects of moxibustion heavily relied on heat-sensitization response, a specific sensation induced by moxibustion in the ill body. On the sensation, long-term potentiation (LTP) of prelimbic cortex was attributed to heat-sensitization responses. The N-methyl-D-aspartic acid (NMDA) receptor plays a key role in LTP induction; however, little is known about the role of NMDA receptor in heat-sensitization response. The present study investigated the role of NMDA receptor in heat-sensitization response, specifically, NMDA receptor was inhibited by competitive glutamatergic antagonist, (±)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP), observing the frequency of heat-sensitization response in moxibustion treatment and evaluating the conducive outcomes to cerebral infarct rats for rehabilitation. Heat-sensitization response in cerebral infarct rats was regularly measured for all the samples when exposed to moxibustion. Intraperitoneal injection of CPP was conducted, and soon afterwards, a significant drop of heat-sensitization response in all the samples was measured. Moreover, moxibustion efficiency on rehabilitation was unfavourably affected in cerebral infarct rats when compared to vehicle injection control. This indicated that NMDA receptor antagonist made a negative impact on induction of heat-sensitization response and consequently affected cerebral infarct rats to rehabilitate under moxibustion treatment. It also suggested that activating NMDA receptor played a positive part in ischemic stroke rehabilitation, and regulating its activity could be a feasible way to increase heat-sensitization response, improving the effect of moxibustion.
我们之前的研究表明，艾灸的效果在很大程度上依赖于热敏化反应，这是艾灸在患病机体中诱导产生的一种特殊感觉。关于这种感觉，前边缘皮层的长时程增强（LTP）被认为归因于热敏化反应。N-甲基-D-天冬氨酸（NMDA）受体在LTP的诱导中起关键作用；然而，关于NMDA受体在热敏化反应中的作用却知之甚少。本研究调查了NMDA受体在热敏化反应中的作用，具体而言，通过竞争性谷氨酸能拮抗剂（±）-3-（2-羧基哌嗪-4-基）丙基-1-膦酸（CPP）抑制NMDA受体，观察艾灸治疗中热敏化反应的频率，并评估对脑梗死大鼠康复的有益效果。当对所有样本进行艾灸时，定期测量脑梗死大鼠的热敏化反应。进行腹腔注射CPP，随后不久，测量到所有样本的热敏化反应显著下降。此外，与注射溶剂对照组相比，脑梗死大鼠的艾灸康复效果受到不利影响。这表明NMDA受体拮抗剂对热敏化反应的诱导产生负面影响，从而影响脑梗死大鼠在艾灸治疗下的康复。这也表明激活NMDA受体在缺血性中风康复中起积极作用，调节其活性可能是增加热敏化反应、提高艾灸效果的一种可行方法。
