SINAE, Sevilla, España.
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, University of Campinas, Campinas, Brazil.
Eur J Contracept Reprod Health Care. 2022 Jun;27(3):199-207. doi: 10.1080/13625187.2021.1975270. Epub 2021 Sep 6.
To analyse the effect of ulipristal acetate (UPA) as emergency contraception (EC) on the gene expression of human endometrial cell line (HEC-1A) and endometrium from fertile women treated with UPA after ovulation.
HEC-1A cells were treated with UPA, and endometrial tissue from four healthy women was collected in cycles before, during and 2 months after post-ovulation pill intake. Ovulation and luteal phase were monitored, and endometrial biopsies were obtained at day LH + 7 in each cycle. In all cases, we analysed the expression profile of 192 genes associated to endometrial receptivity.
We observed a significant change in total transcriptomic activity of UPA-treated HEC-1A cells compared to controls. we also observed a trend to down-regulation of genes in the UPA-treated cycle that was partially restored in the post-treatment cycle. Altogether, our results supported a partially reversible effect of UPA in gene expression associated with uterine receptivity.
When UPA was administered after ovulation, it seems to induce a down-regulation of the main genes involved in conditioning the endometrium for implantation. This effect is partially restored two months after pill intake. The action of UPA on the endometrium for users of EC should be further investigated.
分析屈螺酮（UPA）作为紧急避孕药（EC）对人子宫内膜细胞系（HEC-1A）和排卵后接受 UPA 治疗的生育期妇女子宫内膜基因表达的影响。
用 UPA 处理 HEC-1A 细胞，在排卵后避孕药摄入前、期间和 2 个月后，从 4 名健康女性的子宫内膜组织中采集。监测排卵和黄体期，并在每个周期的 LH + 7 天获得子宫内膜活检。在所有情况下，我们分析了与子宫内膜容受性相关的 192 个基因的表达谱。
与对照组相比，我们观察到 UPA 处理的 HEC-1A 细胞的总转录组活性发生了显著变化。我们还观察到 UPA 处理周期中基因表达下调的趋势，这种趋势在治疗后周期中部分恢复。总之，我们的结果支持 UPA 对与子宫容受性相关的基因表达具有部分可逆的影响。
当 UPA 在排卵后给药时，它似乎会下调参与子宫内膜着床条件的主要基因。这种作用在停药后两个月内部分恢复。应该进一步研究 UPA 对 EC 用户子宫内膜的作用。
