Department of Gynecology and Obstetrics, St. Marien Hospital Amberg, Amberg, Germany.
Institute for Molecular and Cellular Anatomy, University of Regensburg, Regensburg, Germany.
Clin Hemorheol Microcirc. 2021;79(1):157-166. doi: 10.3233/CH-219113.
Breast cancer is the most common malignant tumor in women and highly heterogeneous with a variety of different molecular subtypes. The analysis of the individual tumor biology is necessary to develop a specific and individualized treatment plan for every patient. The chick chorioallantoic membrane (CAM) model, a 3D-in-vivo-tumor-model, could potentially provide a methodology that facilitates the gain of additional information regarding the tumor biology as well as the testing of the tumor's individual sensitivity to different therapies.
The objective was to establish the grafting of different breast cancer primaries onto the CAM for tumor profiling and the investigation of different parameters.
Breast cancer primary tissue of different patients was grafted onto the CAM. Subsequently, 3D volume and perfusion measurements were performed during the engraftment period. Histological analyses of the tumors were carried out after the engraftment period.
The grafting of the breast cancer primaries onto the CAM was successful. The tumors remained partially vital and displayed angiogenic development on the CAM.
Breast cancer primary material can be grafted onto the CAM and we observed visible and measurable changes of perfusion over time.
乳腺癌是女性最常见的恶性肿瘤，具有高度异质性，存在多种不同的分子亚型。分析肿瘤的个体生物学特性对于为每位患者制定特定和个体化的治疗方案是必要的。鸡胚尿囊膜（CAM）模型是一种 3D 体内肿瘤模型，它可能提供一种方法，有助于获得有关肿瘤生物学的更多信息，并测试肿瘤对不同治疗方法的个体敏感性。
本研究旨在将不同的乳腺癌原发灶移植到 CAM 上，以进行肿瘤分析，并研究不同的参数。
将不同患者的乳腺癌原发组织移植到 CAM 上。随后，在移植期间进行 3D 体积和灌注测量。移植后进行肿瘤的组织学分析。
乳腺癌原发灶成功移植到 CAM 上。肿瘤在 CAM 上仍然部分存活，并显示出血管生成的发展。
可以将乳腺癌原发组织移植到 CAM 上，并且我们观察到灌注随着时间的推移而发生可见和可测量的变化。
