Portolés A, Márquez E
Centro de Investigaciones Biológicas, U.E.I. de Immunofarmacología, C.S.I.C., Madrid, España.
Rev Esp Fisiol. 1987 Dec;43(4):503-13.
A microcomputer program (NKCELPRO) which expedites the exact quantification of various parameters referring to NK cell activities is described. The program is written in BASIC language and computes the activities of cell-mediated cytotoxicity by collating and reducing radioactive count data obtained from 51Cr releasing assays. The information generated by this program includes statistics such as the average values, fitting by last square regression line with its correlation coefficient, as well as determination of the percent specific lysis and lytic units/10(7) effector cells according to any reference value previously defined by the operator. Special program features allow for a certain flexibility in the experimental protocol design as well as in the graphic expression of the results. Data storage on a disk with filing purposes for subsequent studies is also available.
本文描述了一个微型计算机程序（NKCELPRO），该程序可加速对各种与自然杀伤（NK）细胞活性相关参数的精确量化。该程序用BASIC语言编写，通过整理和处理从51Cr释放试验获得的放射性计数数据来计算细胞介导的细胞毒性活性。该程序生成的信息包括统计数据，如平均值、用最小二乘回归线拟合及其相关系数，以及根据操作员预先定义的任何参考值确定特异性裂解百分比和裂解单位/10(7)效应细胞。该程序的特殊功能在实验方案设计以及结果的图形表达方面具有一定的灵活性。还可以将数据存储在磁盘上以便存档，供后续研究使用。
