Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Medical Biochemistry Department, Faculty of Human Medicine, Zagazig University, Zagazig, Egypt.
Mol Biol Rep. 2021 Nov;48(11):7095-7104. doi: 10.1007/s11033-021-06699-x. Epub 2021 Sep 6.
Obesity and diabetes prevalence are increasing worldwide. We aimed to detect the possible association of osteoprotegerin (OPG) gene expression with visceral adiposity indices and cardiometabolic risk factors among obese women.
The study enrolled 150 controls and 150 obese cases subdivided into two subgroups non-diabetic (n = 70) and 80 patients with type 2 diabetes mellitus (T2DM). Circulating OPG gene expression levels were figured out by real time PCR (Polymerase Chain Reaction). Serum OPG levels were assessed by Enzyme Linked Immunosorbent Assay. Our results explored that OPG serum levels were lower in the obese women compared to control group (p < 0.001) and obese diabetics had higher serum levels of OPG in comparison to obese non-diabetic patients (p < 0.001). Expression levels of OPG were higher in obese women than controls (p < 0.001). Moreover, the blood expression levels of OPG gene were higher in diabetic obese patients than non-diabetics. We found positive correlations between parameters of metabolic syndrome and obesity indices. After adjustment of the traditional risk factors, stepwise linear regression analysis test revealed that OPG expression levels were independently correlated with glycated hemoglobin, high-density lipoprotein-cholesterol, and waist-to-hip ratio.
OPG mRNA levels were associated with surrogate markers of insulin resistance in Egyptian obese women.
肥胖和糖尿病的患病率在全球范围内呈上升趋势。我们旨在检测肥胖女性的骨保护素 (OPG) 基因表达与内脏脂肪指数和心血管代谢危险因素之间的可能关联。
本研究纳入了 150 名对照者和 150 名肥胖患者，分为非糖尿病（n=70）和 80 名 2 型糖尿病患者（T2DM）两个亚组。通过实时 PCR（聚合酶链反应）测定循环 OPG 基因表达水平。通过酶联免疫吸附试验（ELISA）测定血清 OPG 水平。我们的研究结果表明，与对照组相比，肥胖女性的血清 OPG 水平较低（p<0.001），且肥胖糖尿病患者的血清 OPG 水平高于肥胖非糖尿病患者（p<0.001）。与对照组相比，肥胖女性的 OPG 表达水平更高（p<0.001）。此外，糖尿病肥胖患者的 OPG 基因血表达水平高于非糖尿病患者。我们发现代谢综合征的参数与肥胖指数之间存在正相关。在调整传统危险因素后，逐步线性回归分析显示，OPG 表达水平与糖化血红蛋白、高密度脂蛋白胆固醇和腰臀比独立相关。
OPG mRNA 水平与埃及肥胖女性的胰岛素抵抗替代标志物相关。
