Hameed Basma B, Ismail Zainab Z
Ministry of Education, Baghdad, Iraq.
Department of Environmental Engineering, University of Baghdad, Baghdad, Iraq.
Prep Biochem Biotechnol. 2022;52(5):561-565. doi: 10.1080/10826068.2021.1972427. Epub 2021 Sep 6.
Reactive azo dyes, if discharged in unmonitored manner to natural water bodies, can cause remarkable irreversible damage. The current study is centered on the decolorization and biodegradation of reactive blue (RB4) azo dye in an integrated sequential anaerobic-aerobic batch mode process. The biodegradation of reactive blue (RB4) was accomplished using alternatively both starch and gelatin-immobilized mixed cells. Activated sludge freshly collected from a sewage treatment plant was used as the biocatalyst. Complete decolorization of 10 mg/L RB4 under anaerobic conditions observed within 30 h by using individually mixed bacterial cells immobilized with; (1) starch cross-linked with polyvinyl alcohol (PVA-St beads), and (2) gelatin cross-linked with polyvinyl alcohol (PVA-Ge beads). The results revealed that maximum removal efficiencies of chemical oxygen demand (COD) under aerobic conditions were 89, 88, and 86%, using PVA-St beads, whereby they were 90, 85, 84% using PVA-Ge beads occurred within 70, 90 and 100 h at detected concentrations of 10, 20, and 40 mg/L, respectively. The effect of biocarrier has been proven to be negligible as the results were comparable using both bio-carriers.
活性偶氮染料若未经监测就排放到天然水体中，会造成显著的不可逆损害。当前的研究聚焦于以厌氧 - 好氧间歇式集成顺序模式处理工艺对偶氮活性蓝（RB4）染料进行脱色和生物降解。使用淀粉和明胶固定化混合细胞交替实现了活性蓝（RB4）的生物降解。取自污水处理厂的新鲜活性污泥用作生物催化剂。在厌氧条件下，通过单独使用固定在以下两种物质上的混合细菌细胞，在30小时内观察到10毫克/升RB4完全脱色：（1）与聚乙烯醇交联的淀粉（PVA - St珠），以及（2）与聚乙烯醇交联的明胶（PVA - Ge珠）。结果表明，在好氧条件下，使用PVA - St珠时化学需氧量（COD）的最大去除效率分别为89%、88%和86%，而使用PVA - Ge珠时，在检测浓度分别为10毫克/升、20毫克/升和40毫克/升的情况下，在70小时、90小时和100小时内的去除效率分别为90%、85%和84%。由于使用两种生物载体的结果具有可比性，已证明生物载体的影响可忽略不计。
