College of Agriculture, Northeast Agricultural University, Harbin 150036, PR China.
College of Life Science, Northeast Agricultural University, Harbin 150036, PR China.
J Invertebr Pathol. 2021 Oct;185:107657. doi: 10.1016/j.jip.2021.107657. Epub 2021 Sep 4.
Bacillus thuringiensis (Bt) has been used globally as a biopesticide for effective and environmentally friendly pest control. Research has intensified following the development of resistance by lepidopteran species to Bt insecticidal crystal proteins. Discovering new Bt strains with novel toxin properties which can overcome resistance is one of the strategies to improve pesticide sustainability. The genome of the Bacillus thuringiensis LTS290 strain was sequenced and assembled in 252 contigs containing a total of 6,391,328 bp. The novel cry79Aa1 gene from this strain was identified and cloned. Cry79Aa1 contains 729 amino acid residues and a molecular mass of 84.8 kDa by SDS-PAGE analysis. Cry79Aa1 was found to be active against the lepidopteran larvae of Spodoptera exigua, Helicoverpa armigera, and Plutella xylostella with LC values of 13.627 µg/mL, 42.8 µg/mL, and 38.086 µg/mL, respectively. However, Cry79Aa1 protein showed almost no insecticidal activity against Leguminivora glycinivorella, although some degree of growth retardation was observed.
苏云金芽孢杆菌（Bt）已在全球范围内用作生物农药，用于有效且环保的害虫防治。随着鳞翅目物种对 Bt 杀虫晶体蛋白产生抗性，研究工作得到了加强。发现具有新毒素特性的新型 Bt 菌株，可以克服抗性，是提高农药可持续性的策略之一。Bt LTS290 菌株的基因组被测序并组装成 252 个连续体，总共包含 6391328bp。从该菌株中鉴定并克隆了新型 cry79Aa1 基因。Cry79Aa1 通过 SDS-PAGE 分析含有 729 个氨基酸残基和 84.8kDa 的分子量。Cry79Aa1 对斜纹夜蛾、棉铃虫和小菜蛾的鳞翅目幼虫表现出活性，LC 值分别为 13.627μg/mL、42.8μg/mL 和 38.086μg/mL。然而，Cry79Aa1 蛋白对豆荚野螟几乎没有杀虫活性，尽管观察到一定程度的生长迟缓。
