Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Madrid, Spain.
Sci Rep. 2021 Sep 6;11(1):17692. doi: 10.1038/s41598-021-97245-z.
Transcriptional plant responses are an important aspect of herbivore oviposition studies. However, most of our current knowledge is derived from studies using Lepidopteran models, where egg-laying and feeding are separate events in time. Little is known regarding plant response to pests where females feed and oviposit simultaneously. The present study characterized oviposition-induced transcriptomic response of Arabidopsis to Tetranychus urticae egg extracts. Transcriptional evidence indicates that early events in plant response to the egg extract involve responses typical to biotic stresses, which include the alteration in the levels of Ca and ROS, the modification of pathways regulated by the phytohormones jasmonic acid and ethylene, and the production of volatiles and glucosinolates as defence mechanisms. These molecular changes affect female fertility, which was significantly reduced when mites fed on plants pre-exposed to the egg extract. However, longer periods of plant exposure to egg extract cause changes in the transcriptional response of the plant reveal a trend to a decrease in the activation of the defensive response. This alteration correlated with a shift at 72 h of exposition in the effect of the mite feeding. At that point, plants become more susceptible and suffer higher damage when challenged by the mite.
植物的转录反应是研究植食性昆虫产卵的一个重要方面。然而，我们目前的大部分知识都来自于使用鳞翅目模型的研究，在这些研究中，产卵和取食是时间上分开的两个事件。对于雌性同时取食和产卵的情况下植物对害虫的反应，我们知之甚少。本研究描述了拟南芥对桃蚜卵提取物的产卵诱导转录组反应。转录证据表明，植物对卵提取物早期反应的早期事件涉及对生物胁迫的典型反应，包括 Ca 和 ROS 水平的改变、受植物激素茉莉酸和乙烯调控的途径的修饰，以及挥发物和硫代葡萄糖苷的产生作为防御机制。这些分子变化会影响雌性的生殖力，当螨虫在预先接触过卵提取物的植物上取食时，雌性的生殖力会显著降低。然而，植物长时间暴露于卵提取物会导致植物转录反应的变化，显示出防御反应激活程度降低的趋势。这种变化与螨取食 72 小时时的作用变化相关。在这一点上，当受到螨虫的攻击时，植物变得更容易受到侵害，并遭受更高的损害。
