Department of Ophthalmology and Visual Science, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Methods Mol Biol. 2022;2549:307-320. doi: 10.1007/7651_2021_418.
We have established a stepwise method to differentiate induced pluripotent stem cells (iPSCs) into retinal pigment epithelium (RPE) (iPSC-RPE), which enables efficient isolation and purification of patient-derived iPSC-RPE cells with high quality. Here, we describe in detail the process of differentiating iPSCs into iPSC-RPE.
我们已经建立了一种逐步的方法，将诱导多能干细胞（iPSCs）分化为视网膜色素上皮（RPE）（iPSC-RPE），这使得高效分离和纯化具有高质量的患者来源的 iPSC-RPE 细胞成为可能。在这里，我们详细描述了将 iPSCs 分化为 iPSC-RPE 的过程。
