Zhao Wenli, Wang Kun, Chang Yanpeng, Zhang Bo, Li Fei, Meng Yuxuan, Li Mengqi, Zhao Quanzhi, An Shiheng
College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
College of Agronomy, Henan Agricultural University, Zhengzhou, China.
Plant Cell Environ. 2022 Mar;45(3):900-914. doi: 10.1111/pce.14180. Epub 2021 Sep 17.
Plant root morphology is constantly reshaped in response to triggers from the soil environment. Such modifications in root system architecture involve changes in the abundance of reactive oxygen species (ROS) in the apoplast and in cell wall (CW) composition. The hybrid proline-rich proteins (HyPRPs) gene family in higher plants is considered important in the regulation of CW structure. However, the functions of HyPRPs remain to be characterized. We therefore analysed the functions of OsR3L1 (Os04g0554500) in rice. qRT-PCR and GUS staining revealed that OsR3L1 is expressed in roots. While the r3l1 mutants had a defective root system with fewer adventitious roots (ARs) and lateral roots (LRs) than the wild type, lines overexpressing OsR3L1 (R3L1-OE) showed more extensive LR formation but with a shorter root length. The expression of OsR3L1 was initiated by the OsMADS25 transcription factor. Moreover, the abundance of OsR3L1 transcripts was increased by NaCl. The R3L1-OE-3 line exhibited enhanced salt tolerance, whereas the r3l1-2 mutant showed greater salt sensitivity. The addition of H O increased the levels of OsR3L1 transcripts. Data are presented indicating that OsR3L1 modulates H O accumulation in the apoplast. We conclude that OsR3L1 regulates salt tolerance through regulation of peroxidases and apoplastic H O metabolism.
植物根系形态会不断根据土壤环境中的触发因素进行重塑。根系结构的这种改变涉及质外体中活性氧（ROS）含量的变化以及细胞壁（CW）组成的改变。高等植物中的富含脯氨酸的杂交蛋白（HyPRPs）基因家族被认为在细胞壁结构的调节中起重要作用。然而，HyPRPs的功能仍有待确定。因此，我们分析了水稻中OsR3L1（Os04g0554500）的功能。定量逆转录聚合酶链反应（qRT-PCR）和β-葡萄糖苷酸酶（GUS）染色显示OsR3L1在根中表达。与野生型相比，r3l1突变体的根系存在缺陷，不定根（ARs）和侧根（LRs）较少，而过表达OsR3L1的株系（R3L1-OE）侧根形成更广泛，但根长较短。OsR3L1的表达由OsMADS25转录因子启动。此外，NaCl可增加OsR3L1转录本的丰度。R3L1-OE-3株系表现出增强的耐盐性，而r3l1-2突变体表现出更高的盐敏感性。添加过氧化氢（H₂O₂）可增加OsR3L1转录本的水平。现有数据表明，OsR3L1调节质外体中H₂O₂的积累。我们得出结论，OsR3L1通过调节过氧化物酶和质外体H₂O₂代谢来调节耐盐性。
