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MADS盒转录因子OsMADS25通过影响水稻中硝酸盐积累来调控根系发育。

MADS-box transcription factor OsMADS25 regulates root development through affection of nitrate accumulation in rice.

作者信息

Yu Chunyan, Liu Yihua, Zhang Aidong, Su Sha, Yan An, Huang Linli, Ali Imran, Liu Yu, Forde Brian G, Gan Yinbo

机构信息

Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.

College of Life Sciences, Zhejiang University, Hangzhou, China.

出版信息

PLoS One. 2015 Aug 10;10(8):e0135196. doi: 10.1371/journal.pone.0135196. eCollection 2015.

DOI:10.1371/journal.pone.0135196
PMID:26258667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4530940/
Abstract

MADS-box transcription factors are vital regulators participating in plant growth and development process and the functions of most of them are still unknown. ANR1 was reported to play a key role in controlling lateral root development through nitrate signal in Arabidopsis. OsMADS25 is one of five ANR1-like genes in Oryza Sativa and belongs to the ANR1 clade. Here we have investigated the role of OsMADS25 in the plant's responses to external nitrate in Oryza Sativa. Our results showed that OsMADS25 protein was found in the nucleus as well as in the cytoplasm. Over-expression of OsMADS25 significantly promoted lateral and primary root growth as well as shoot growth in a nitrate-dependent manner in Arabidopsis. OsMADS25 overexpression in transgenic rice resulted in significantly increased primary root length, lateral root number, lateral root length and shoot fresh weight in the presence of nitrate. Down-regulation of OsMADS25 in transgenic rice exhibited significantly reduced shoot and root growth in the presence of nitrate. Furthermore, over-expression of OsMADS25 in transgenic rice promoted nitrate accumulation and significantly increased the expressions of nitrate transporter genes at high rates of nitrate supply while down-regulation of OsMADS25 produced the opposite effect. Taken together, our findings suggest that OsMADS25 is a positive regulator control lateral and primary root development in rice.

摘要

MADS盒转录因子是参与植物生长发育过程的重要调节因子，其中大多数的功能仍不清楚。据报道，ANR1在拟南芥中通过硝酸盐信号控制侧根发育方面起关键作用。OsMADS25是水稻中五个类ANR1基因之一，属于ANR1进化枝。在这里，我们研究了OsMADS25在水稻对外部硝酸盐响应中的作用。我们的结果表明，OsMADS25蛋白在细胞核和细胞质中均有发现。在拟南芥中，OsMADS25的过表达以硝酸盐依赖的方式显著促进了侧根和主根的生长以及地上部的生长。在硝酸盐存在的情况下，转基因水稻中OsMADS25的过表达导致主根长度、侧根数量、侧根长度和地上部鲜重显著增加。转基因水稻中OsMADS25的下调在硝酸盐存在的情况下表现出地上部和根部生长显著减少。此外，在转基因水稻中OsMADS25的过表达促进了硝酸盐积累，并在高硝酸盐供应速率下显著增加了硝酸盐转运蛋白基因的表达，而OsMADS25的下调则产生相反的效果。综上所述，我们的研究结果表明，OsMADS25是水稻中控制侧根和主根发育的正向调节因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/8b65c1ccd107/pone.0135196.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/8ee5f5770f5e/pone.0135196.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/88f9cec8ab8e/pone.0135196.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/3bbac873d6af/pone.0135196.g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/8bcee7740562/pone.0135196.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/e5826480fc50/pone.0135196.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/b1d99c369ae7/pone.0135196.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/c758ee04ae13/pone.0135196.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/49c31d775026/pone.0135196.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/8b65c1ccd107/pone.0135196.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/8ee5f5770f5e/pone.0135196.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/3bbac873d6af/pone.0135196.g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/8bcee7740562/pone.0135196.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/e5826480fc50/pone.0135196.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/b1d99c369ae7/pone.0135196.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/c758ee04ae13/pone.0135196.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/49c31d775026/pone.0135196.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62f5/4530940/8b65c1ccd107/pone.0135196.g010.jpg

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