Kumari Poonam, Bowmik Sujoy, Paul Sudipto Kumar, Biswas Bidisha, Banerjee Sanjay K, Murty Upadhyayula Surayanarayana, Ravichandiran Velayutham, Mohan Utpal
Department of Biotechnology, National Institute of Pharmaceutical Education & Research (NIPER), Guwahati, Assam, India.
Department of Medicinal Chemistry, National Institute of Pharmaceutical Education & Research (NIPER), Kolkata, West Bengal, India.
Biotechnol Bioeng. 2021 Dec;118(12):4577-4589. doi: 10.1002/bit.27935. Epub 2021 Sep 27.
Sortase A, a transpeptidase enzyme is present in many Gram-positive bacteria and helps in the recruitment of the cell surface proteins. Over the last two decades, Sortase A has become an attractive tool for performing in vivo and in vitro ligations. Sortase A-mediated ligation has continuously been used for its specificity, robustness, and highly efficient nature. These properties make it a popular choice among protein engineers as well as researchers from different fields. In this review, we give an overview of Sortase A-mediated ligation of various molecules on the cell surfaces, which can have diverse applications in interdisciplinary fields.
分选酶A是一种转肽酶，存在于许多革兰氏阳性细菌中，有助于募集细胞表面蛋白。在过去二十年中，分选酶A已成为体内和体外连接的一种有吸引力的工具。分选酶A介导的连接因其特异性、稳健性和高效性而被持续使用。这些特性使其成为蛋白质工程师以及不同领域研究人员的热门选择。在本综述中，我们概述了分选酶A介导的细胞表面各种分子的连接，这在跨学科领域可能有多种应用。
