Pang P K, Harvey S, Doris P A
Department of Pharmacology and Therapeutics, Texas Tech University Health Sciences Center, Lubbock 79430.
Can J Physiol Pharmacol. 1987 Dec;65(12):2386-9. doi: 10.1139/y87-378.
Plasma parathyroid hormone levels (pPTH) have been measured by radioimmunoassay (RIA) in young spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto controls (WKY) aged from 6 to 16 weeks to assess the possible role of PTH during the development of hypertension. Three antisera were used in the RIAs. One antiserum was directed toward the inactive C-terminal fragment of PTH, another toward the bioactive N-terminal fragment (PTH 1-34), and a third was obtained by immunization against intact PTH 1-84. Blood pressures were measured by tail-cuff plethysmography with prewarming. Blood ionized calcium and sodium concentrations (b[Ca2+] and b[Na+]) were determined by ion-selective electrolyte analysis. No significant differences were observed between pPTH in the SHR compared with WKY during the development of hypertension. Neither were significant differences in b[Ca2+] or b[Na+] present at any age. The expected progression of hypertension in SHRs was observed and blood pressure was significantly greater in SHR than in WKY at all times. The results suggest that differences in pPTH and b[Ca2+] in SHR reported in other studies may be secondary phenomena to the establishment of hypertension. Our data suggest that PTH is not involved in the pathogenetic processes occurring during the development of spontaneous hypertension in rats.
采用放射免疫分析法（RIA）测定了6至16周龄的年轻自发性高血压大鼠（SHR）和正常血压的Wistar-Kyoto对照大鼠（WKY）的血浆甲状旁腺激素水平（pPTH），以评估PTH在高血压发展过程中的可能作用。放射免疫分析中使用了三种抗血清。一种抗血清针对PTH的无活性C末端片段，另一种针对生物活性N末端片段（PTH 1-34），第三种是通过用完整的PTH 1-84免疫获得的。通过预热后采用尾套体积描记法测量血压。通过离子选择性电解质分析测定血离子钙和钠浓度（b[Ca2+]和b[Na+]）。在高血压发展过程中，SHR的pPTH与WKY相比未观察到显著差异。在任何年龄，b[Ca2+]或b[Na+]也均无显著差异。观察到SHR中高血压的预期进展，并且在所有时间SHR的血压均显著高于WKY。结果表明，其他研究报道的SHR中pPTH和b[Ca2+]的差异可能是高血压形成的继发现象。我们的数据表明，PTH不参与大鼠自发性高血压发展过程中发生的致病过程。
