Mechanism of contractile action of barium ion on rat aortic smooth muscle.

The mechanism of Ba2+-induced contraction has been examined in helical strips of Ca2+-depleted, 60 mM K+-depolarized rat aortae. The concentration-response curves to Ca2+ or Ba2+ were significantly potentiated by exposure to 3 X 10(-8) M Bay K 8644 (a Ca2+ channel agonist) in the order Ca2+ greater than Ba2+, suggesting an action of Ba2+ ions through potential-sensitive membrane Ca2+ channels. Exposure of strips to background concentration of Ca2+ (0.05 mM) enhanced the contractile responses to Ba2+, whereas background exposure to Ba2+ (0.1 mM) attenuated Ca2+ responses. Repeated stimulation with Ba2+ resulted in tachyphylaxis, contrary to the result when Ca2+ was used. The results suggest that Ba2+ ions enter rat aortic smooth muscle cells through Ca2+ channels and mobilize a noradrenaline-insensitive intracellular Ca2+ pool. Ba2+ may also cause a desensitization of some intracellular process.