Center of General Surgery, The 80th Group Army Hospital of People's Liberation Army, Weifang, China.
State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
Phlebology. 2022 Feb;37(1):63-71. doi: 10.1177/02683555211043332. Epub 2021 Sep 8.
Superficial venous thrombosis (SVT) is the complications of varicose great saphenous veins (VGSVs), but its pathogenesis remains unclear. This study was designed to measure the changes in expression of matrix metalloproteinases (MMPs) and the tissue inhibitor of metalloproteinases (TIMPs) from SVT, VGSVs, and great saphenous veins (GSVs).
In the venous walls of the three groups, the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 proteins, protein-positive expression ratios, mRNA expression, and protein expression were determined by immunohistochemistry, polymerase chain reaction, and western blot.
The MMP-2, MMP-9, TIMP-1, and TIMP-2 protein-positive expression ratios, mRNA and protein expression in the SVT group were significantly higher than those in the VGSV and the GSV groups. The corresponding expression in the VGSV group were significantly higher than those in the GSV group.
Disequilibrium of MMPs and TIMPs in SVT wall occurs due to underlying high hydrostatic pressure and inflammation. These results suggested that MMPs and TIMPs participate in the process of venous wall remodeling.
浅静脉血栓（SVT）是大隐静脉曲张（VGSV）的并发症，但发病机制尚不清楚。本研究旨在测量 SVT、VGSV 和大隐静脉（GSV）中基质金属蛋白酶（MMPs）和金属蛋白酶组织抑制剂（TIMPs）表达的变化。
通过免疫组织化学、聚合酶链反应和 Western blot 检测三组静脉壁中 MMP-2、MMP-9、TIMP-1 和 TIMP-2 蛋白的表达、蛋白阳性表达率、mRNA 表达和蛋白表达。
SVT 组 MMP-2、MMP-9、TIMP-1 和 TIMP-2 蛋白阳性表达率、mRNA 和蛋白表达均明显高于 VGSV 组和 GSV 组，VGSV 组明显高于 GSV 组。
SVT 壁中 MMPs 和 TIMPs 的失衡是由于潜在的高压和炎症引起的。这些结果表明 MMPs 和 TIMPs 参与了静脉壁重塑的过程。
