Shi Li-Juan, Wang Hui-Min, Fu Xiao-Li, Kou Liang, Meng Sheng-Wang, Dai Xiao-Qin
Qianyanzhou Ecological Research Station, Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Science and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China.
College of Resource and Environment, University of Chinese Academy of Sciences, Beijing 100190, China.
Ying Yong Sheng Tai Xue Bao. 2020 Jun;31(6):1980-1988. doi: 10.13287/j.1001-9332.202006.008.
We measured the activities of six kinds of enzyme, including β-glucosidase (BG), β-N-acetyl-glucosaminidase (NAG), leucine aminopeptidase (LAP), acid phosphatase (AP), polyphenol oxidase (POX), peroxidase (POD), as well as enzyme stoichiometric ratios and soil physical and chemical properties at 0-10 and 10-20 cm layers across typical Pinus massoniana plantation, Pinus elliottii plantation and mixed plantation of P. massoniana and Schima superba (broadleaved-conifer mixed plantation) in mid-subtropical China. Key factors driving the variation in soil enzyme activity and stoichiometry among different stand types were investigated. The results showed that the activities of soil BG and LAP were significantly affected by stand type. Soil BG activity at 10-20 cm soil layer was significantly higher in P. elliottii plantation than in P. massoniana plantation, while the activity of LAP was highest in the P. massoniana plantation. Soil BG/(NAG+LAP) and BG/AP at 10-20 cm layer of P. elliottii plantation were significantly higher than those of P. massoniana plantation, while (NAG+LAP)/AP of P. massoniana plantation was significantly higher than those of P. elliottii plantation and mixed plantation. The vector length of enzyme stoichiometry at 10-20 cm soil layer was significantly different among stand type, with an order of P. elliottii plantation > broadleaved-conifer mixed plantation > P. massoniana. The vector angles of enzyme stoichiometry in the three plantations were greater than 45°, with the vector angle in the P. elliottii plantation at 10-20 cm soil layer being significantly greater than that of the P. massoniana plantation. Results from redundancy analysis showed that soil carbon quality index and the ratio of soil organic carbon to total phosphorus (C/P), soil water content and C/P were the key factors affecting soil enzyme activity and stoichiometry at 0-10 and 10-20 cm soil layers, respectively. The quantity and quality of soil carbon and phosphorus, and soil water content played a key role in regulating nutrient cycling in mid-subtropical plantation ecosystem.
我们在中国亚热带中部典型的马尾松人工林、湿地松人工林以及马尾松与木荷混交林（针阔混交林）的0-10厘米和10-20厘米土层中，测定了六种酶的活性，包括β-葡萄糖苷酶（BG）、β-N-乙酰氨基葡萄糖苷酶（NAG）、亮氨酸氨肽酶（LAP）、酸性磷酸酶（AP）、多酚氧化酶（POX）、过氧化物酶（POD），以及酶化学计量比和土壤理化性质。研究了不同林分类型间土壤酶活性和化学计量比变化的关键驱动因素。结果表明，土壤BG和LAP的活性受林分类型的显著影响。湿地松人工林10-20厘米土层的土壤BG活性显著高于马尾松人工林，而LAP活性在马尾松人工林中最高。湿地松人工林10-20厘米土层的土壤BG/(NAG+LAP)和BG/AP显著高于马尾松人工林，而马尾松人工林的(NAG+LAP)/AP显著高于湿地松人工林和混交林。10-20厘米土层的酶化学计量比向量长度在不同林分类型间存在显著差异，顺序为湿地松人工林>针阔混交林>马尾松人工林。三种人工林的酶化学计量比向量角均大于45°，其中湿地松人工林10-20厘米土层的向量角显著大于马尾松人工林。冗余分析结果表明，土壤碳质量指数和土壤有机碳与总磷的比值（C/P）、土壤含水量和C/P分别是影响0-10厘米和10-20厘米土层土壤酶活性和化学计量比的关键因素。土壤碳和磷的数量与质量以及土壤含水量在调节亚热带人工林生态系统养分循环中起关键作用。
