Crop Biotechnology Research Group, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
Western Australian State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
Methods Mol Biol. 2022;2360:119-138. doi: 10.1007/978-1-0716-1633-8_11.
Next-generation sequencing and analyses of whole-genome transcripts can be used to identify genes and potential mechanisms that may be responsible for the development of resistance to insecticides. Such genes can be identified by isolating and sequencing high-quality messenger RNA and identifying differentially expressed genes (DEGs), and gene variants from insecticide-treated and untreated colonies of the Green peach aphid (GPA) or resistant and susceptible GPA populations. Datasets generated would reveal a set of genes whose expression may be associated with the insecticide treatment. The DEGs can then be validated using quantitative PCR assays.
下一代测序和全基因组转录本分析可用于鉴定可能导致对杀虫剂产生抗性的基因和潜在机制。这些基因可以通过分离和测序高质量的信使 RNA，并从绿桃蚜（GPA）的经杀虫剂处理和未经处理的群体或抗性和敏感的 GPA 群体中鉴定差异表达基因（DEGs）和基因变异来识别。生成的数据集将揭示一组其表达可能与杀虫剂处理相关的基因。然后可以使用定量 PCR 测定法来验证差异表达基因。
