Howard P C, DeMarco G J, Consolo M C, McCoy G D
Department of Environmental Health Sciences, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106.
Mol Toxicol. 1987 Apr-Sep;1(2-3):177-89.
The effect of ethanol consumption by male CF-1 mice on liver microsomal enzyme activities has been investigated. The total microsomal cytochrome P-450 content was increased by 38%, while cytochrome b5 was decreased by 31%, which are characteristic alterations in liver microsomes following ethanol consumption. Other alterations included a decreased NADPH cytochrome c reductase activity and increased NADPH-supported rates of N-nitrosopyrrolidine and aniline hydroxylation. While ethanol consumption did not alter the total metabolism of nicotine, the rates of N- and C-hydroxylation were differently affected. The 5'-hydroxylation of nicotine was increased by 83%, while the N'-oxidation was decreased by 31%. Changes in the microsomal metabolism of the environmental carcinogen 1-nitropyrene included a slight reduction in the overall metabolism, which can be accounted for by a reduction in the formation of one phenolic metabolite, 1-nitropyren-3-ol.
已研究了雄性CF - 1小鼠摄入乙醇对肝脏微粒体酶活性的影响。微粒体细胞色素P - 450的总含量增加了38%，而细胞色素b5减少了31%，这是摄入乙醇后肝脏微粒体的典型变化。其他变化包括NADPH细胞色素c还原酶活性降低，以及N - 亚硝基吡咯烷和苯胺羟基化的NADPH支持率增加。虽然摄入乙醇并未改变尼古丁的总代谢，但N - 羟基化和C - 羟基化的速率受到了不同影响。尼古丁的5'-羟基化增加了83%，而N'-氧化减少了31%。环境致癌物1 - 硝基芘微粒体代谢的变化包括整体代谢略有降低，这可以通过一种酚类代谢物1 - 硝基芘 - 3 - 醇形成的减少来解释。
