Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Chemosphere. 2022 Jan;287(Pt 2):132185. doi: 10.1016/j.chemosphere.2021.132185. Epub 2021 Sep 6.
Hydrogen peroxide (HO) is an environmental-friendly algicide and it is widely used to control algal blooms in aquatic ecosystems. However, the response of algal cell metabolic characteristics and intracellular protein profile under HO stress is still not well understood. In the present study, the green alga Scenedesmus obliquus was exposed to different concentrations of HO (0, 2, 6, 8 and 10 mg L) to evaluate the changes in algal morphological, physiological, and proteomic features to HO exposure. The results showed that 8 mg L of HO could effectively inhibit the cell growth and photosynthetic activity of S. obliquus including chlorophyll-a content and chlorophyll fluorescence parameters. The increased activities of superoxide dismutase (SOD) and catalase (CAT) observed in this study indicate that cells exposure to HO caused oxidative stress. The metabolic activity of S. obliquus was significantly decreased by HO treatment. In terms of proteomic analysis, 251 differentially expressed proteins (DEPs) were successfully identified. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed significant protein enrichment in the metabolic pathways, photosynthesis, ascorbic acid, and alginate metabolism and phenylpropane biosynthesis of S. obliquus. The analysis of protein-protein interaction system shows that the pathways of photosynthesis and metabolic pathways of S. obliquus were essential to resist oxidative stress. Taking together, these results shed new lights on exploring the cell physiological metabolism and intracellular protein mechanisms of HO inhibition on algal blooms.
过氧化氢（HO）是一种环保型杀藻剂，广泛用于控制水生生态系统中的藻类水华。然而，HO 胁迫下藻类细胞代谢特征和细胞内蛋白质谱的响应仍不清楚。本研究中，将斜生栅藻暴露于不同浓度的 HO（0、2、6、8 和 10 mg/L）中，以评估藻类形态、生理和蛋白质组特征对 HO 暴露的变化。结果表明，8 mg/L 的 HO 能有效抑制斜生栅藻的细胞生长和光合作用，包括叶绿素-a 含量和叶绿素荧光参数。本研究中观察到超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性的增加表明细胞受到 HO 引起的氧化应激。HO 处理显著降低了斜生栅藻的代谢活性。在蛋白质组分析方面，成功鉴定出 251 个差异表达蛋白（DEPs）。京都基因与基因组百科全书（KEGG）途径富集分析显示，斜生栅藻的代谢途径、光合作用、抗坏血酸、褐藻酸盐代谢和苯丙烷生物合成途径中存在显著的蛋白质富集。蛋白质-蛋白质相互作用系统的分析表明，光合作用和代谢途径是斜生栅藻抵抗氧化应激的关键途径。总之，这些结果为探索 HO 抑制藻类水华的细胞生理代谢和细胞内蛋白质机制提供了新的思路。
