School of Energy Engineering, Yulin University, Yulin 719000, China.
South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, China.
Molecules. 2021 Aug 26;26(17):5178. doi: 10.3390/molecules26175178.
Four-wave-mixing microscopy is widely researched in both biology and medicine. In this paper, we present a simplified near-degenerate four-wave-mixing microscopy (SNDFWM). An ultra-steep long-pass filter is utilized to produce an ultra-steep edge on the spectrum of a femtosecond pulse, and a super-sensitive four-wave-mixing (FWM) signal can be generated via an ultra-steep short-pass filter. Compared with the current state-of-the-art FWM microscopy, this SNDFWM microscopy has the advantages of simpler experimental apparatus, lower cost, and easier operation. We demonstrate that this SNDFWM microscopy has high sensitivity and high spatial resolution in both nanowires and biological tissues. We also show that the SNDFWM microscopy can achieve an ultra-sensitive detection based on the electron-resonance effect. This method might find an important application in tracking of nano drugs in vivo.
四波混频显微镜在生物学和医学领域都有广泛的研究。在本文中，我们提出了一种简化的近简并四波混频显微镜（SNDFWM）。我们利用超陡长通滤波器在飞秒脉冲的光谱上产生超陡边缘，然后利用超陡短通滤波器产生超灵敏的四波混频（FWM）信号。与目前最先进的 FWM 显微镜相比，这种 SNDFWM 显微镜具有实验装置更简单、成本更低、操作更容易的优点。我们证明了这种 SNDFWM 显微镜在纳米线和生物组织中具有高灵敏度和高空间分辨率。我们还表明，SNDFWM 显微镜可以基于电子共振效应实现超灵敏检测。这种方法可能在体内追踪纳米药物方面有重要应用。
