Chen Xi, Sun Shouxuan, Geng Tianxiang, Fan Xin, Zhang Shifeng, Zhao Sijia, Geng Yi, Jin Qunhua
Department of Orthopedic Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.
Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu 210042, P.R. China.
Exp Ther Med. 2021 Oct;22(4):1119. doi: 10.3892/etm.2021.10553. Epub 2021 Aug 4.
As an activator of sirtuin 1, resveratrol has become an extensively reviewed anti-inflammatory and anti-aging drug in recent years, and it has been widely studied for the treatment of energy control and endocrine diseases. The present study attempted to characterize the role of resveratrol in osteolysis induced by titanium (Ti) alloy particles and Ti pins and . , bone marrow mesenchymal stem cells were cultured with Ti alloy particles to simulate osteolysis. Cell viability and the expression levels of proteins associated with osteogenesis and the Wnt/β-catenin signaling pathway, including Runt-related transcription factor 2 (Runx2), alkaline phosphatase, osteocalcin, β-catenin, lymphoid enhancer-binding factor 1 and transcription factor 4, were increased following treatment with resveratrol after 21 days of osteogenic differentiation. , a Ti pin model in C57BL/6J mice was used to study the anti-osteolysis effect of resveratrol on the peri-prosthetic bone. The pulling force of the Ti alloy pin was increased in a dose-dependent manner in the resveratrol groups compared with the control group. Furthermore, the results of micro-CT scanning revealed that the bone volume and the bone surface/volume ratio in the periprosthetic tissue were increased in the resveratrol-treated groups, particularly in the high-dose resveratrol group. In addition, immunohistochemistry demonstrated that Runx2 expression was upregulated in the high-dose resveratrol group. In conclusion, the results of the present study indicated that resveratrol may inhibit Ti particle-induced osteolysis via activation of the Wnt/β-catenin signaling pathway and .
作为一种沉默信息调节因子1（sirtuin 1）的激活剂，白藜芦醇近年来已成为一种受到广泛综述的抗炎和抗衰老药物，并且已针对能量控制和内分泌疾病的治疗进行了广泛研究。本研究试图阐明白藜芦醇在钛（Ti）合金颗粒和Ti钉诱导的骨溶解中的作用，并且，将骨髓间充质干细胞与Ti合金颗粒共同培养以模拟骨溶解。在成骨分化21天后用白藜芦醇处理后，细胞活力以及与成骨和Wnt/β-连环蛋白信号通路相关的蛋白质表达水平升高，这些蛋白质包括 runt相关转录因子2（Runx2）、碱性磷酸酶、骨钙素、β-连环蛋白、淋巴样增强子结合因子1和转录因子4。此外，使用C57BL/6J小鼠的Ti钉模型来研究白藜芦醇对假体周围骨的抗骨溶解作用。与对照组相比，白藜芦醇组中Ti合金钉的拉力呈剂量依赖性增加。此外，显微CT扫描结果显示，白藜芦醇处理组中假体周围组织的骨体积和骨表面/体积比增加，特别是在高剂量白藜芦醇组中。此外，免疫组织化学表明高剂量白藜芦醇组中Runx2表达上调。总之，本研究结果表明白藜芦醇可能通过激活Wnt/β-连环蛋白信号通路来抑制Ti颗粒诱导的骨溶解。
