Health and Biomedical Innovation, UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.
Centre of Healthy Ageing and Wellness, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
J Gerontol A Biol Sci Med Sci. 2022 Feb 3;77(2):243-249. doi: 10.1093/gerona/glab264.
Significant alterations in sleep duration and/or quality of sleep become more pronounced as people get older. Poor sleep in elderly people is associated with adverse health outcomes and cellular aging. We examined the relationship between telomere length (TL) and sleep duration, Health Promotion Index (HPI), and tested whether the presence of Apolipoprotein-E4 (ApoE-ε4) allele affects both sleep and TL. The present study was carried out in 174 healthy participants (21% male; mean age 53.79 years) from South Australia. Lymphocyte TL was measured by real-time quantitative PCR (qPCR) and ApoE genotype was determined by TaqMan assay. HPI was calculated from a questionnaire regarding 8 lifestyle habits, including sleeping hours. Multivariate regression analysis was used to establish these associations adjusted for specified confounders. TL was found to be inversely associated with age (r = -0.199; p = .008) and body mass index (r = -0.121; p = .11), and was significantly shorter in participants who slept for less than 7 hours (p = .001) relative to those sleeping ≥7 hours. TL was positively correlated with HPI (r = 0.195; p = .009). ApoE-ε4 allele carriers who slept for less than 7 hours had shortest TL (p = .01) compared to noncarriers. Plasma soluble receptor for advanced glycation end product (sRAGE) level was significantly (p = .001) lower in individuals who sleep less than 7 hours and ApoE-ε4 carriers. Our results suggest that inadequate sleep duration or poor HPI is associated with shorter TL in cognitively normal people and that carriage of APOE-ε4 genotype may influence the extent of these effects.
随着年龄的增长，睡眠持续时间和/或睡眠质量的显著变化变得更加明显。老年人睡眠质量差与不良健康结果和细胞衰老有关。我们研究了端粒长度 (TL) 与睡眠持续时间、健康促进指数 (HPI) 的关系，并测试了载脂蛋白 E4 (ApoE-ε4) 等位基因的存在是否会同时影响睡眠和 TL。本研究在来自南澳大利亚的 174 名健康参与者（21%为男性；平均年龄 53.79 岁）中进行。通过实时定量 PCR (qPCR) 测量淋巴细胞 TL，通过 TaqMan 测定确定 ApoE 基因型。HPI 是通过关于 8 种生活方式习惯（包括睡眠时间）的问卷计算得出的。使用多元回归分析来建立这些关联，并根据指定的混杂因素进行调整。TL 与年龄呈负相关 (r = -0.199;p =.008) 和体重指数 (r = -0.121;p =.11)，与睡眠时间少于 7 小时的参与者相比，睡眠时间少于 7 小时的参与者 TL 明显较短 (p =.001)。TL 与 HPI 呈正相关 (r = 0.195;p =.009)。睡眠时间少于 7 小时且携带 ApoE-ε4 等位基因的携带者 TL 最短 (p =.01) 与非携带者相比。睡眠时间少于 7 小时且携带 ApoE-ε4 基因型的个体的血浆可溶性晚期糖基化终产物受体 (sRAGE) 水平显著降低 (p =.001)。我们的研究结果表明，认知正常人群中，睡眠持续时间不足或 HPI 较低与 TL 较短有关，APOE-ε4 基因型的携带可能会影响这些影响的程度。
