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通过离子交换色谱法从大鼠大脑皮层中分离毒蕈碱型乙酰胆碱受体实体。

Separation of muscarinic acetylcholine receptor entities from rat cerebral cortex by ion exchange chromatography.

作者信息

Pelzer H, Raible A

机构信息

Thomae Research Laboratories, Department of Biochemistry, Biberach/Riss, FRG.

出版信息

J Recept Res. 1987;7(6):845-57. doi: 10.3109/10799898709054565.

DOI:10.3109/10799898709054565
PMID:3450870
Abstract

Digitonin solubilized muscarinic acetylcholine receptor (mAChR) of rat cerebral cortex membranes were chromatographed on the FPLC anion exchanger Mono Q. [3H]QNB (quinuclidinyl benzilate) and [3H]PZ (pirenzepine) binding activity was retarded from a NaCl free elution buffer and thereby separated from a part of the accompanying proteins. Elution of the column with a continuously increasing NaCl concentration desorbed the radioligand binding activities forming several peaks, two of which were nearly completely separated. Our data show that the mAChR in rat cerebral cortex consists of several entities with different electrical charges.

摘要

用洋地黄皂苷增溶的大鼠大脑皮层膜毒蕈碱型乙酰胆碱受体（mAChR）在快速蛋白质液相色谱（FPLC）阴离子交换剂Mono Q上进行层析。[3H]QNB（喹核醇基苯酸酯）和[3H]PZ（哌仑西平）的结合活性在无氯化钠洗脱缓冲液中被阻滞，从而与一部分伴随蛋白分离。用不断增加氯化钠浓度的溶液洗脱柱子，可解吸附放射性配体结合活性，形成几个峰，其中两个峰几乎完全分离。我们的数据表明，大鼠大脑皮层中的mAChR由几个带不同电荷的实体组成。

相似文献

1
Separation of muscarinic acetylcholine receptor entities from rat cerebral cortex by ion exchange chromatography.通过离子交换色谱法从大鼠大脑皮层中分离毒蕈碱型乙酰胆碱受体实体。
J Recept Res. 1987;7(6):845-57. doi: 10.3109/10799898709054565.
2
Characterization of [3H]pirenzepine binding to muscarinic cholinergic receptors solubilized from rat brain.[3H]哌仑西平与从大鼠脑中溶解的毒蕈碱型胆碱能受体结合的特性研究。
J Pharmacol Exp Ther. 1985 Jul;234(1):37-44.
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Solubilization with digitonin alters the kinetics of pirenzepine binding to muscarinic receptors from rat forebrain and heart.用洋地黄皂苷增溶会改变哌仑西平与大鼠前脑和心脏毒蕈碱受体结合的动力学。
J Pharmacol Exp Ther. 1987 Sep;242(3):981-90.
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Solubilization and characterization of high and low affinity pirenzepine binding sites from rat cerebral cortex.大鼠大脑皮层中高亲和力和低亲和力哌仑西平结合位点的增溶及特性研究
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[3H]pirenzepine and (-)-[3H]quinuclidinyl benzilate binding to rat cerebral cortical and cardiac muscarinic cholinergic sites. I. Characterization and regulation of agonist binding to putative muscarinic subtypes.[3H]哌仑西平和(-)-[3H]奎宁环基苯甲酸酯与大鼠大脑皮质和心脏毒蕈碱胆碱能位点的结合。I. 激动剂与假定毒蕈碱亚型结合的特性及调节
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6
[3H]pirenzepine and (-)-[3H]quinuclidinyl benzilate binding to rat cerebral cortical and cardiac muscarinic cholinergic sites. II. Characterization and regulation of antagonist binding to putative muscarinic subtypes.[3H]哌仑西平和(-)-[3H]东莨菪碱与大鼠大脑皮质及心脏毒蕈碱胆碱能位点的结合。II. 拮抗剂与假定毒蕈碱亚型结合的特性及调节
J Pharmacol Exp Ther. 1986 May;237(2):419-27.
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Differential ontogeny of putative M1 and M2 muscarinic receptor binding sites in the murine cerebral cortex and heart.小鼠大脑皮层和心脏中假定的M1和M2毒蕈碱受体结合位点的差异个体发育。
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Heterogeneity of solubilized muscarinic cholinergic receptors: binding and hydrodynamic properties.可溶性毒蕈碱胆碱能受体的异质性：结合与流体动力学特性
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Thermodynamic analyses of pirenzepine binding to membrane-bound and solubilized muscarinic receptors from rat forebrain and heart.哌仑西平与大鼠前脑和心脏的膜结合型及可溶性毒蕈碱受体结合的热力学分析。
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Antibodies to a synthetic peptide can be used to distinguish between muscarinic acetylcholine receptor binding sites in brain and heart.针对合成肽的抗体可用于区分大脑和心脏中的毒蕈碱型乙酰胆碱受体结合位点。
Mol Pharmacol. 1988 Sep;34(3):327-33.