Oshima M, Miyamoto I, Takaoka T, Goto K, Kagawa Y
Division of Clinical Biochemistry, National Medical Center, Tokyo, Japan.
Jpn J Exp Med. 1987 Dec;57(6):351-4.
In cultured cells, essential lipids are usually supplied by the serum added to the culture medium. However, in a serum-free medium, essential lipids would need to be synthesized by the cells. Cell strains derived from kidney, spleen, uterine carcinoma and fibroblasts were cultured in serum-free synthetic media for more than 10 years and their fatty acids were analyzed capillary column gas-liquid-chromatography. The following conclusions were drawn: 1. Polyunsaturated fatty acids were not detected. Thus, prostaglandins are not appear to be essential for initiation of DNA synthesis in these cells. 2. A compensatory increase of the content of monounsaturated fatty acids was detected. 3. A large difference in vaccenic/oleic acid ratio was found among several strains of cells, but the ratio does not reflect that of the original tissues.
在培养细胞中，必需脂质通常由添加到培养基中的血清提供。然而，在无血清培养基中，必需脂质需要由细胞合成。从肾脏、脾脏、子宫癌和纤维母细胞衍生的细胞株在无血清合成培养基中培养了10多年，并通过毛细管柱气液色谱法分析了它们的脂肪酸。得出以下结论：1. 未检测到多不饱和脂肪酸。因此，前列腺素似乎对这些细胞中DNA合成的起始并非必需。2. 检测到单不饱和脂肪酸含量的代偿性增加。3. 在几种细胞株中发现了痘苗酸/油酸比率的巨大差异，但该比率并不反映原始组织的比率。
