Silva Alessandra C G, Silva-Torres Christian S A, Nascimento Deividy V, Torres Jorge B
Departamento de Agronomia - Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900 Recife, PE, Brazil.
Departamento de Agronomia - Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900 Recife, PE, Brazil.
Behav Processes. 2021 Nov;192:104500. doi: 10.1016/j.beproc.2021.104500. Epub 2021 Sep 10.
Ladybird beetles present as key predator of many agricultural pests. Among them, Eriopis connexa stands out due some important traits for pest management programs such as common occurrence, population selected for resistance to pyrethroid insecticides, and possibility to be commercially available by rearing using alternative prey. Despite that, little is known about the role of polyandry and its effect on paternity in this species. Ladybird beetles engage in multiple matings, raising questions about the benefits of polyandry and paternity. We studied the selection of mating pair, sperm precedence, and offspring paternity by performing experiments on (1) the age of sexual maturity, (2) the frequency and refractory mating behavior within a photophase period, (3) the preference of insecticide-susceptible females to mate with either susceptible or resistant male phenotypes; and (4) the effect of multiple matings, on progeny paternity. Sexual maturity in E. connexa was found to begin within 3 days of adult emergence for both sexes, but fully developed after 5 days. The highest frequency of mating exhibited by sexual mature pairs occurred within first hour of pairing and the phenotype for insecticide resistance did not affect the choice of a mating partner. The resistance trait marker in the resistant beetle phenotype indicates that progeny paternity results from a mixture of available sperm and do not depend on mating order. These data suggest that released beetles would have a simple prospect for progeny production for both insecticide resistant and susceptible beetles.
瓢虫是许多农业害虫的主要捕食者。其中，康氏粉瓢虫因其在害虫管理计划中的一些重要特性而脱颖而出，比如常见、对拟除虫菊酯类杀虫剂具有抗性的种群，以及通过使用替代猎物饲养实现商业供应的可能性。尽管如此，对于该物种中一妻多夫制的作用及其对父权的影响却知之甚少。瓢虫会进行多次交配，这引发了关于一妻多夫制和父权益处的问题。我们通过对以下方面进行实验，研究了交配配偶的选择、精子优先性和后代父权：（1）性成熟年龄；（2）光周期内的交配频率和不应期交配行为；（3）对杀虫剂敏感的雌性与敏感或抗性雄性表型交配的偏好；以及（4）多次交配对后代父权的影响。结果发现，康氏粉瓢虫两性在成虫羽化后3天内开始性成熟，但5天后才完全发育成熟。性成熟的配对在配对后的第一小时内交配频率最高，并且杀虫剂抗性表型不影响交配伴侣的选择。抗性甲虫表型中的抗性性状标记表明，后代父权来自可用精子的混合，并不取决于交配顺序。这些数据表明，对于抗杀虫剂和对杀虫剂敏感的甲虫来说，释放的甲虫在后代繁殖方面前景简单。
