Laboratory of Genetics, University of Wisconsin-Madison, Madison, Wisconsin, USA,
Laboratory of Genetics, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Cytogenet Genome Res. 2021;161(8-9):463-469. doi: 10.1159/000516998. Epub 2021 Sep 10.
Meiotic recombination affects fertility, shuffles genomes, and modulates the effectiveness of natural selection. Despite conservation of the recombination pathway, the rate of recombination varies among individuals and along chromosomes. Recombination rate also differs among cells from the same organism, but this form of variation has received less attention. To identify patterns that characterize intercellular variation in the genome-wide recombination rate, we counted foci of the MLH1 recombination-associated protein in oocytes and spermatocytes from a panel of wild-derived inbred strains of house mice. Females show higher intercellular variation in MLH1 focus count than males from the same inbred strains. This pattern is consistent across strains from multiple subspecies, including 2 strains in which the average MLH1 focus count is higher in males. The sex difference in genome-wide recombination rate we report suggests that selection targeting recombination rate will be more efficient in males than in females.
减数分裂重组影响生育能力、打乱基因组并调节自然选择的有效性。尽管重组途径具有保守性，但重组率在个体之间和染色体上存在差异。来自同一生物体的细胞之间的重组率也存在差异，但这种形式的变异受到的关注较少。为了确定表征基因组范围内重组率细胞间变异的模式，我们在一系列来自野生近交系的小家鼠的卵母细胞和精母细胞中计算了 MLH1 重组相关蛋白的焦点。与来自同一近交系的雄性相比，雌性的 MLH1 焦点计数具有更高的细胞间变异性。这种模式在多个亚种的多个品系中都是一致的，包括 2 个雄性 MLH1 焦点计数较高的品系。我们报告的全基因组重组率的性别差异表明，针对重组率的选择在雄性中比在雌性中更有效。
