Saxena Ashok Kumar, Khrolia Deepanshu, Chilkoti Geetanjali T, Gondode Prakash Gyandev, Sharma Tusha, Thakur Gaurav, Banerjee Basu Dev
Department of Anesthesiology and Critical Care, University College of Medical Sciences and Guru Teg Bahadur Hospital, Maharashtra, India.
Department of Anesthesiology and Critical Care, All India Institute of Medical Sciences, Nagpur, Maharashtra, India.
Indian J Palliat Care. 2021 Apr-Jun;27(2):251-256. doi: 10.25259/IJPC_339_20. Epub 2021 Aug 12.
The aim of this study is to study the modulation of extracellular signal-regulated protein kinase (ERK) and tissue inhibitors of matrix metalloproteases 1 (TIMP 1) gene in patients with neuropathic pain (NP).
In the present, cross-sectional, observational study, 2 ml of venous baseline sample was withdrawn from all the patients with neuropathic (NP) or non NP (NNP) soon after their diagnosis or on their first visit to the pain clinic. A real-time quantitative polymerase chain reaction experiment was conducted to measure the mRNA expression of TIMP1 and ERK genes in blood samples. The Delta Ct, Delta Ct, and fold change analysis of both the genes were conducted between patients with NP and NNP.
A total of 285 patients with chronic pain were assessed, out of which, 153 patients had NP and 132 had NNP. The average duration of chronic pain was 11 months for 285 patients. The mRNA expression of TIMP1 gene is significantly down regulated (2.65-fold) ( (-f. 01), and the mRNA expression level of ERK is significantly up regulated (2.03-fold) ( (-f. 01) in NP patients when compared with NNP.
The mRNA expression of TIMP1 gene is significantly down regulated, and ERK is significantly up regulated in patients with NP. Further, multicentric trials with larger sample size are recommended to confirm this finding.
本研究旨在探讨神经病理性疼痛（NP）患者细胞外信号调节蛋白激酶（ERK）和基质金属蛋白酶组织抑制剂1（TIMP-1）基因的调节情况。
在本次横断面观察性研究中，所有神经病理性疼痛（NP）或非神经病理性疼痛（NNP）患者在确诊后或首次就诊于疼痛门诊时，即刻采集2ml静脉基线样本。进行实时定量聚合酶链反应实验，以测量血样中TIMP-1和ERK基因的mRNA表达。对NP患者和NNP患者的这两个基因进行ΔCt、ΔΔCt和倍数变化分析。
共评估了285例慢性疼痛患者，其中153例为NP患者，132例为NNP患者。285例患者的慢性疼痛平均持续时间为11个月。与NNP患者相比，NP患者中TIMP-1基因的mRNA表达显著下调（2.65倍）（P<0.01），ERK的mRNA表达水平显著上调（2.03倍）（P<0.01）。
NP患者中TIMP-1基因的mRNA表达显著下调，ERK显著上调。此外，建议进行更大样本量的多中心试验以证实这一发现。
